| Department of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College. Beijing, 100005, 'People's Republic of China.The present study investigated systematically the cell growth, differentiation and the ultrastructure of the nuclear matrix-intermediate filament system of the cybrid cells, K-RRneo, cybridized between the neo gene transferred rabbit reticulocytes and the human erythroleukemia K562 cells, results indicated:1. With the employment of lipofectin gene transfer techniques, the rabbit reticulocytes were used as the anucleus target cells for research and has been proved to be the suitable host cells for appropriately recombinant foreign gene expression. The reticulocytes, though deprived of nucleus, still retained almost all the cellular organelles necessary for the translation of transcribed mRNA. The reporter gene Lac Z, inserted in an expression vector with promoters and enhancers, could be expressed in the cytoplasm of rabbit reticulocytes. This method provided a valuable model system for cybrid cells selection with reliable genetic markers and for the study of erythroid differentiation, denucleation, nuclear reconstitution as well as the mechanism of EDF action.2. A cybrid cell strain (designated as K-RRneo) of G418 resistance was established by fusion of the rabbit reticulocytes transferred with pSV2neo plasmid and the human erythroleukemia K562 cells. The method consisted of the procedure of lipofectin gene transfer instead of the tedious and time consuming prerequisite of selecting HGPRT deficient mutant in traditional method, and with a set of...
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