| In order to reveal the different differentiation of cellular structures and characteristics on the condition of resting state in ciliates, the vegetative cell and resting cyst in Urostyla grandis were used as experimental materials. By using the methods of selective extraction, SDS-PAGE, DGD embedding and embedment-free transmission electron microscope (TEM) and TEM, a comparative study on ultrastructure and intermediate-type filament -lamina-nuclear matrix system and its protein compositions of the vegetative cell and resting cyst in Urostyla grandis were carried.1. UltrastructureAfter the formation of resting cyst in Urostyla grandis, the cyst wall is composed of three layers named from outside to inside: ectocyst, endocyst, and granular layer. Ciliary shaft and microtubule are absorbed by cortex , except for part of the kinetosome. It is proved that cyst wall undergo the process of becoming thinner and thinner and break at last, the ciliatures grow gradually from the position of kinetosomes and adoral zone of membranelle(AZM) is formed during the resting cyst's differentiation . It is found that autophagic vacuoles include almost integrated paraglycodens, mitochondriae and membrane structures, some ones are attached around by many ribosomes, during the resting cyst's differentiation. The inner membrane system becomes complex gradually, The net structure possibly developing into endoplasmic reticulum and Golgi body, form accordingly, during the resting cyst's differentiation. A lot of mitochondriae become bigger, its outer and inner membrane become vague, the cristae in them also become vague, sometimes they will converge, in the resting cyst, these phenomena will disappear during the resting cyst's differentiation. With a great of observation to the macronuclear's shapes, It is obtained that some macronuclears display the shape of dumb-bell, some macronuclears expand in their middle and become thinner between middle and two ends. So the macronuclears perhaps have two ways of divisions - two divisions and three divisions at least, It is possible that the macronuclears recover their number using the two ways. No obvious changes happen in the micronuclear's shape, which perhaps have no relation with the process of resting cyst's differentiation. These results provide documents for more study on the mechanism of cellular resting and differentiation in ciliates.2. Intermediate-type filament-lamina-nuclear matrix system and its protein compositionsThe intermediate-type filament-lamina-nuclear matrix system is left in the vegetative cell and resting cyst of Urostyla grandis, whose entirety structure andbasic shape unit are similar, and whose basic shape unit is made up with single filaments that diameter is 10 nm or with filament bundle that are combined by single filaments. The area equal to the nuclear in the cell, is maintained by the lamina, while the filaments of the lamina connect the nuclear matrix filaments and the intermediate-type filaments in cytoplasm(CIFs), and the CIFs end at the scaffold which locate at the edge of cell and are combined with many filaments. The three kinds of filaments make an organic entirety, and build up a continue and stable net system in cell. Talking it from the morphology, the net system plays a role of frame, and is concerned with the cellular organs location, which is also an important factor to keep cellular structure integrated. The stability of the intermediate-type filament-lamina-nuclear matrix system in ciliates suggests that it perhaps plays an important role in ciliates cells.The protein compositions of intermediate-type filament-lamina-nuclear matrix system change after the formation of resting cyst in Urostyla grandis: there are 29 protein bands of the system in the vegetative cell and 24 protein bands in resting cyst, the former and the latter have 4 and 1 specific bands respectively, both have 23 similar bands. Concerning as the protein compositions, almost protein compositions of intermediate-type filament-lamina-nuclear matrix system i...
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