Correlation Between Vaginal Microecology And High-Risk HPV Infection

ObjectiveReview the relevant indicators of vaginal micro-ecology of patients with high risk human papilloma virus（HR-HPV）infection of the cervix,analyze the factors that affect the infection and prognosis of HR-HPV of the cervix,with a view to improving the vaginal micro-ecology,improving the clearance rate of HR-HPV,reducing the incidence of cervical lesions caused by the continuous infection of HR-HPV,and effectively preventing the occurrence of cervical cancer.MethodsFrom September 2020 to September 2021,300 women were selected from the gynecological clinic of the First Affiliated Hospital of Hebei North for cervical APTIMA HPV test,cervical TCT exfoliative cytology test and vaginal micro-ecological microscopy.（1）Study 1:180 women who were found to be infected with HR-HPV for the first time without cervical lesions were selected as the study group,and 120 women who were negative for HR-HPV and cervical TCT exfoliative cytology in the same period were selected as the control group.Compare the differences of vaginal micro-ecological indicators between the study group and the control group:pH value,cleanliness,white blood cell number,fungi（VVC）,trichomonas（TV）,clue cells（BV）,sialidase（SNA）,white blood cell esterase（LE）and hydrogen peroxide（H₂O₂）.（2）Study 2:The study group was followed up for 6 and 12 months to analyze the negative status of HR-HPV and explore the relationship between the negative status of HR-HPV and vaginal microecology.Results（1）Study 1:（1）In the study group and the control group,there was no statistical difference in age between the two groups（P>0.05）;（2）Single factor analysis in the two groups:vaginal pH,H₂O₂ and BV were significantly different between the two groups（P<0.05）;（3）A single factor was included in a multivariate logistic regression analysis,and the results showed that H₂O₂ and BV were independent risk factors for HR-HPV infection.（2）Study 2:（1）The negative rate of HR-HPV in the cervix at 6 and 12months of follow-up:there was statistically significant difference in age between the negative and non-negative groups(P Single factor analysis of vaginal micro-ecology:In the negative and non-negative groups,there were statistically significant differences in vaginal pH,cleanliness,white blood cell count,LE and H₂O₂ between the two groups（P<0.05）;There was no significant difference in VVC,TV,BV and SNA between the two groups（P>0.05）;（3）The factors with P<0.05 in the single factor analysis were included in the multivariate logistic regression analysis.The results showed that age,pH value,cleanliness and H₂O₂ were independent risk factors for the conversion of HR-HPV to negative;（4）There was no significant difference between HR-HPV negative and different HR-HPV types（P>0.05）.The other 11 high-risk HPV positive groups had the highest negative rate（82.5%,82.6%）,and the HPV16 type had the lowest negative rate（7.5%,6.5%）;（5）At 6 and 12 months follow-up,the negative rate of HR-HPV infection was 22.2%and 40.4%respectively.Conclusion1.Disorder of vaginal micro-ecology affects cervical HR-HPV infection and negative conversion.2.H₂O₂ deficiency and BV positive are independent risk factors of HR-HPV infection;Age>50 years old,increased pH value,decreased cleanliness and lack of H₂O₂ are independent risk factors affecting the conversion of HR-HPV to negative.