Study On Safety Evaluation Of Commonly Used Medicinal And Edible Substances Based On Three-dimensional Coupling Method

Objective(1)Based on the " three-dimensional coupling " research idea,a three-dimensional database of the potential toxicity of " ingredients,acting target organs,and organism toxicity " of medicinal and edible substances is built,using the principle of database and computer technology,to provide detailed data support for the study of the potential toxicity mechanism of medicinal and edible substances,and to assist the deep development of the study of safety evaluation of medicinal and edible substances.(2)Fused the " three-dimensional coupling " research mode with computer map visualization technology to explore the potential toxic action mechanisms of each medicinal and edible substance,and to construct maps of corresponding action relationships.(3)The Analytic Hierarchy process(AHP)was used to construct computer models for safety evaluation of medicinal and edible substances and explore the possibility of computer technology in the analysis and evaluation of safety of medicinal and edible substances.Methods(1)Database construction methodThe relevant data search strategy and inclusion and screening criteria were developed and searched in the CTD database(Comparative Toxicogenomics Database),TCMSP(Traditional Chinese Medicine Systems pharmacology Database and Analysis Platform)database,Biogps database(http://biogps.org)And CNKI,WANFANG DATA and VIP Database were retrieved to extract the data of the three aspects--" ingredients,action target organs,and organism toxicity " of commonly used medicinal and edible substances,and the data were managed and applied by using office2021 Excel software and Medicinal and Edible Substance Safety Evaluation Platform(autonomous R & D).(2)Atlas visualization methodThis subject has adopted computer technology such as Python language and Pye Charts to visualize the relationship between the potential toxic "three-dimensional" data of medicinal and edible substances,and in the form of a relationship map,the potential toxic mechanism of various medicinal and edible substances was exhibited.(3)Analytic hierarchy processBased on the large amount of data from the three-dimensional database of existing medicinal and edible substances,using operational ideas of Analytical Hierarchy Process(AHP),through the steps of literature survey,expert consultation,determining the indicators and hierarchical relationships,evaluation information collection,consistency testing,and obtaining evaluation weights,the AHP model for safety evaluation of medicinal and edible substances was constructed,obtained the results of computer safety evaluation of food and drug substances based on objective study data,and analyzed and discussed.Results(1)Complete the establishment of the toxic components-target database,toxic target organ database and body toxicity database(referred to as "three-dimensional" database)of commonly used medicinal and edible substances.Among them,the toxic component-target database contains 25 "component-target" data of medicinal and edible substances,151 ingredients with clear toxic effects on human body,and a test set containing 28 toxic Chinese herbal ingredients.The toxic target organ database contains 25 "acting target organs" data of drug substances and4203 target organ information.The body toxicity database contains 24 "animal toxicity" data of medicinal and edible substances,24 "adverse reactions" data of medicinal and edible substances,and 405 terms of adverse reactions.(2)Through visual analysis of the "three-dimensional coupling" atlas of the "three-dimensional" data of the medicinal and edible substances,the "three-dimensional coupling" atlas of 22 medicinal and edible substances was drawn,among which,14 medicinal and edible substances had clear "three-dimensional" toxicity relationship;The reports of adverse reactions of eight medicinal and edible substances mostly involve allergic reactions,drug eruptions and dermatitis,but the obtained "three-dimensional" atlas still lacks key links.(3)Using the established AHP model,the safety of 30 kinds of medicinal and edible substances and a positive control toxic drug-aconite was ranked and evaluated.The preliminary safety ranking was as follows: Crocus sativus,Prunella vulgaris,Platycodon grandiflorum,Aconite,Cape jasmine,Ginkgo biloba,Eucommia ulmoides leaves,Cassia seed,Honey,Turmeric,Houttuynia cordata,Lilium,Siraitia grosvenorii,Ginseng,Gastrodia elata,Cornus officinalis,Coix seed,Lycii Fructus,Licorice,Amomum villosum,Dendrobium officinale,Poria,Lotus seed,White lentils,Cistanche deserticola,Ganoderma lucidum,Astragalus,Pueraria,Raspberry,Malt,Yam.The lower the order,the higher the safety.Conclusions(1)The construction of a "three-dimensional" database of commonly used medicinal and edible substances,on the one hand,is conducive to the accumulation of a large number of basic research data of medicinal and edible substances,convenient for query and application development research;On the other hand,it is conducive to the collection and sharing of research data on the safety of medicinal and edible substances,and to the research and development of network toxicology and potential toxicity mining of medicinal and edible substances.(2)The drawing results of the "three-dimensional coupling" potential toxic effect relationship map show that: on the one hand,the "three-dimensional coupling" evaluation model proposed in this study has good feasibility,which can provide a new research idea for the safety evaluation of medicinal and edible substances or the safety evaluation of traditional Chinese medicine;On the other hand,the three-dimensional coupling potential toxicity relationship map of medicinal and edible substances provided in this study shows the main potential toxicity relationships under the current basic research data,which is convenient for researchers to quickly understand or roughly trace the possible or existing toxic effects in the study,and promote the development of research related to the safety of medicinal and edible substances.(3)The research ideas of safety evaluation of traditional Chinese medicine and appropriate computer algorithms can be well integrated to complete the evaluation task together.By building a more sophisticated and multi-level computer evaluation model,a large number of Chinese medicine research data can be summarized and analyzed,providing researchers with more comprehensive and objective safety evaluation results,and providing certain reference value for the follow-up in-depth construction of a new Chinese medicine safety evaluation system.