| Objective: To observe the effects of Tianxing Jianshenwei Granules on brain neurotransmitters in D-galactose-induced aging rats,and to provide scientific basis for the effects of Tianxing Jianshenwei granules on brain aging and the development of health care products.Methods: 60 male SD rats were randomly divided into 6 groups with 10 rats per group according to their body weight: blank group,model group,vitamin E group,Tianxing Jianshenwei granules low-dose,medium-dose and high-dose groups,respectively.In this study,the aging model was established by intraperitoneal injection of D-GAL.Rats in model group,vitamin E group,Tianxing Jianshenwei Granule low-dose,medium-dose and high-dose groups were injected with D-GAL solution intraperitoneally every day,according to the standard of 0.5g/kg,and the model was constructed for 6 weeks(42 days).From the 3rd week(15th day)of modeling,Tianxing Jianshenwei Granules low-dose,medium-dose and high-dose groups were administered according to the standard of 2.7g/kg,5.4g/kg and 10.8g/kg,respectively;vitamin E group was given intragastric administration according to the standard of 27mg/kg;blank group and model group were given intragastric administration of normal saline with the same volume ratio,once a day.A total of 4 weeks(28 days)were given by intragastric administration.The Morris Water Maze experiment was conducted for 5 days after administration.After the experiment,the body mass of all the rats was weighed for the last time,and the brain tissue of the rats was quickly and completely removed from the ice box after anesthesia.The hypothalamus was stained with HE to observe the morphological changes of the hippocampus.The double antibody sandwich method was used to conduct enzyme linked immunoassay to determine the content of Ach and the activity of ChAT and ACh E in the brain tissue of the rats.Content of monoamine transmitters DA,NE,5-HT,NO content and activity of NOS.Results:1.Morris Water maze test results of rats: Compared with blank group,average latency of rats in model group was significantly increased(P<0.01),average swimming speed and times of crossing platform area were significantly decreased(P<0.01);Compared with model group,the average latency of rats in high-dose group and vitamin E group was significantly decreased(P<0.01),the average swimming speed of rats in high-dose group and vitamin E group was significantly increased(P<0.01),and The Times of crossing the platform area in high-dose group,medium-dose group and vitamin E group was significantly increased(P<0.01 or P<0.05).The average latency period,average swimming speed and times of crossing the platform area were not significantly changed in the low-dose group(P>0.05),and there was no statistical significance.2.The observation results of HE staining in hippocampus tissue were as follows: blank group: the somatic cells of hippocampus were arranged neatly,the cytoplasmic nuclei in cells were clearly stained,the morphology of neurons was regular,there was no glial cell proliferation,no edema and congestion,and no inflammatory cells.Model group: hippocampal somatic cells were disorganized,cytoplasmic nucleus boundaries were not clear,nuclei shrank,neurons were not arranged properly,the number of glial cells and inflammatory cells increased,and cells showed hyperemia and edema.Vitamin E group,low-dose group,medium-dose group,and high-dose group:Compared with model group,the morphological changes of hippocampus were improved,which were mainly manifested as more orderly cell arrangement,less intracellular nuclear melting,and fewer microglia and inflammatory cells.The improvement was not obvious in low-dose group,slightly better in medium-dose group,and the improvement was most obvious in high-dose group and vitamin E group.3.Ach content in brain tissue of rats,activity indexes of ChAT and AchE were determined: compared with blank group,Ach content in brain tissue of rats in model group was significantly decreased(P<0.01);Compared with model group,Ach content in brain tissue of rats in high-dose group and vitamin E group was significantly increased(P<0.01),that in medium-dose group was significantly increased(P<0.05),and that in low-dose group was not significantly increased(P>0.05).Compared with blank group,ChAT activity of brain tissue in model group was significantly decreased(P<0.01).Compared with model group,ChAT activity in brain tissue of rats in Tianxing Jianshenwei granule high-dose,medium-dose and low-dose groups and vitamin E groups was significantly increased(P<0.01).Compared with blank group,AchE activity in brain tissue of model group was significantly increased(P<0.01).Compared with model group,AchE activity in brain tissue of rats in Tianxing Jianshenwei granule high-dose,medium-dose and low-dose groups and vitamin E groups was significantly decreased(P<0.01).4.The contents of monoamine transmitters DA,NE and 5-HT in brain tissue of rats were determined as follows: compared with blank group,the contents of DA,NE and 5-HT in brain tissue of rats in model group were significantly decreased(P<0.01);Compared with model group,the contents of DA,NE and 5-HT in brain tissue of rats in Tianxing Jianshenwei granule high,medium and low dose groups and vitamin E groups were significantly increased(P<0.01 or P<0.05).5.Determination results of NO content and NOS activity in brain tissue of rats: compared with blank group,NO content and NOS activity in brain tissue of rats in model group were significantly increased(P<0.01);Compared with model group,NO content and NOS activity in brain tissue of rats in high-dose group,medium-dose group and vitamin E group were significantly decreased(P<0.01 or P<0.05),while NO content and NOS activity in low-dose group were not significantly decreased,with no statistical significance(P>0.05).Conclusion:Tianxingjianshenwei granules can improve the learning and memory ability and the morphology of hippocampus in aging rats.ACh content was increased,ChAT activity was increased,AchE activity was decreased;Increase the contents of monoamine transmitters DA,NE and 5-HT in brain tissue of aging rats;Reduce the content of NO and the activity of NOS in the brain tissue of aging rats,so as to play a role in delaying brain aging. |
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