The Effect Of Saussurea Medusa Maxim Polysaccharide On Reducing UVB Induced Skin Injury In Mice Through Aquaporin-3 Pathway

Objective To investigate the protective effect of Saussurea medusa Maxim polysaccharide on UVB induced skin injury in mice,and to lay the theoretical foundation for its clinical application.Methods Thirty Kunming male mice were randomly divided into three groups,Normal group,UVB group and UVB+ Saussurea medusa group.Mice in UVB group and UVB+ Saussurea medusa group were injected with 5% D-galactose 10ml/(kg·d)subcutaneously and subjected to UVB irradiation for 40 d continuously.Mice in Normal group as fed under normal light and was subcutaneously injected with normal saline of the same volume every day.Starting from the 11 th day of modeling,mice in UVB+ Saussurea medusa group was gavaged with a dose of 4g/(kg·d),mice in Normal group and UVB group were gavaged with normal saline of the same volume daily respectively.After 30 days,skin histomorphological variation were observed,skin moisture content was measured,and expressions of epidermal growth factor receptor(EGFR),extracellular regulatory protein kinase(ERK),and aquaporin 3(AQP3)in AQP3 pathway were detected.Results Under light microscope,compared with UVB group,the epidermis of UVB+ Saussurea medusa group was thinner,the dermis was thicker,and the fibrous tissue was more densely arranged and orderly.The expression of EGFR and ERK in UVB group was higher than that in Normal group and the expression of AQP3 was lower than that in normal group(P<0.05).The expression of EGFR and ERK in UVB+ Saussurea medusa group were lower than those in UVB group and the expression levels of AQP3 were higher than those in UVB group(P<0.05).Conclusion The polysaccharide of Saussurea medusa Maxim can alleviate the skin injury of mice caused by UVB through AQP3 pathway.