Study On The Preparation And Pharmacodynamics Of Tang Shen Kang(Concentrated) Pill

Objective:Through the research on the preparation process of Tangshenkang(concentrate)pill,the best technology for the extraction of Tangshenkang(concentrate)pill was optimized,on this basis,the key technical parameters of the molding process of the concentrated pills were investigated,and the traditional Chinese medicine Tangsikang(concentrate)pill was made.Combined with the clinical indications of the drug,the pharmacodynamics experimental study on the mechanism of Tangshenkang(concentrate)pill in thetreatment of diabetic nephropathy was conducted.Methods:(1)Using orthogonal test experiment method,the weights of astragaloside Ⅳ extract and extract yield are comprehensive evaluation indicators.The decocting time、decocting times and water addition times were investigated for optimizing the extraction process parameters of Astragali radix and other medicinal materials.Using single factor combined with orthogonal test method,dissolution time,roundness,pellet rate as evaluation indexes,the size of the powder crushing,Pan-injection mold powder and Pan-time when the amount of water were investigated,and optimized the preparation process of Tang Shen Kang(concentrated)pill,and the process verification is carried out.(2)Study on Pharmacodynamics of Tang Shenkang(Concentrate)Pill.The model of diabetic nephropathy was induced by unilateral nephrectomy and hyperlipidemia and high glucose diet.The rats were randomly divided into sham operation group,benner pury group,model group,high dose,medium and low doses of Tang Shen Kang.After administration,the GLU,Hb A1c,TG,TC,U Alb,Scr,BUN values were determined for each group.To investigate the therapeutic effect of Tangshenkang(concentrate)pill on diabetic nephropathy(DN).Result:(1)Take 1/2 of Astragalus membranaceus in prescription,mix with yam,Poria,Atractylodes,Chuanxiong and Fructus Mume into fine powder for standby.The rest of the medicine was added 8 times water,decocted for 2 times,2 hours each time,filtered,combined with filtrate,concentrated to the relative density of 1.20-1.30(60 C)thick paste,mix with the above fine powder,dry,crushed into fine powder,spare;take 14%of the amount of powder,from the mold,Pan pill,dry,made of Tang Shen Kang(concentrate)pill;(2)Tang Shen Kang(Concentrate)Pills had different degrees of improvement in GLU,HbAlc,TG,TC,UAlb,Scr,and BUN in DN model rats,and the administration group was statistically significant compared with the model group.,showing a significant difference(P<0.05).Conclusion:Through the resarch of Tang Shen Kang pill’s(concentrated pill)preparation process and pharmacodynamic comparative,it is proved that the preparation process is stable,reasonable and feasible,which can be used in preclinical trials of new drugs and provide experimental basic data for new drug applications.