| Objective: Both Zheng’s No.1 new injury medicine and ice compress are clinically used to treat“damaged blood stasis syndrome”,which have the characteristics of good curative effect and quick effect.This study compared the effects of the two treatments and observed the effects of the two treatments on the expression and tissue structure of IL-1,IL-10 and TNF-α in skeletal muscle of rats.The possible mechanism provides a certain theoretical and experimental reference for the clinical treatment of injured blood stasis syndrome through the discussion of the treatment mechanism.Methods: Seventy-eight male SD rats were randomly divided into four groups: blank group,model control group,Zheng’s No.1 new injury medicine group,and ice pack group.The last three groups were divided into four groups: 1D,3D,5D,7D.The phases are divided into 4 groups(n = 6).The blank group was routinely fed,and the remaining groups were modeled by "quantitative gravity strike method".The model control group was not intervened,and the Zheng’s No.1 new injury drug group and the ice pack group each intervened.Rats were sacrificed at corresponding phases,specimens were collected,and the expressions of IL-1,IL-10 and TNF-α in skeletal muscle were detected by Elisa method and observed under pathological microscope.Data processing and analysis were performed using SPSS 23.0 statistical software.Result: 1.Body weight: The body weight of each group was lower than that of the blank group,and there was no statistically significant difference between the groups(P> 0.05).2.The circumference of the affected limb: Compared with the blank group,1D after modeling,each group increased significantly(P <0.01);3D after modeling,the control group increased significantly(P <0.05).3.Skeletal muscle IL-1 concentration:(1)Compared with the blank group: 1D and 3D after modeling,the control group and ice pack group significantly increased(P <0.01);5D after modeling: the control group significantly increased(P <0.01),The ice group increased significantly(P <0.05).(2)Compared with the model-building control group: 1D,3D,and 5D after model-building,the new injury drug group was significantly reduced(P <0.05).(3)Comparison of intervention group: 3D after modeling,the new injury drug group was lower than the ice pack group(P <0.05).4.Skeletal muscle IL-10 concentration:(1)Compared with the blank group: 1D after modeling,each group significantly increased(P <0.01);3D,5D,7D after modeling,the control group and ice group significantly increased(P <0.01).(2)Compared with the model-building control group: 1D,5D,7D after modeling,the new wounded medicine group was significantly reduced(P <0.05);3D after modeling,the new wounded medicine group was significantly reduced(P <0.01).(3)Comparison of intervention group: 3D,5D,7D after modeling,the new wound medicine was lower than ice pack(P <0.01).5.Skeletal muscle TNF-α concentration:(1)Compared with the blank group: 1D and 3D after modeling,each group increased significantly(P <0.01);5D after modeling,the model control group significantly increased(P <0.01),and the new injury drug group significantly increased High(P <0.05);7D after modeling,the model control group increased significantly(P <0.01).(2)Compared with the model control group: 1D after modeling,the new wound medicine group and ice pack group were significantly reduced(P <0.01);3D after modeling,the new wound medicine group was significantly reduced(P <0.05),ice pack Group was significantly reduced(P <0.01);5D after modeling,the iced group was significantly reduced(P <0.05);7D after modeling,the iced group was significantly reduced(P <0.01).(3)Comparison of intervention groups: no significant difference(P> 0.05).6.Pathological tissue section: Compared with the blank group,1D after modeling,the skeletal muscle tissue of the control group and the new injury drug group was significantly damaged;3D after modeling,the skeletal muscle tissue of the control group and the new injury drug group was lightly damaged.The other groups of skeletal muscle tissue were basically normal.Conclusion: 1.Zheng’s new medicine No.1 and ice compress have obvious curative effect on rats with "damage blood stasis syndrome",which can effectively improve the symptoms and signs of injury,and promote the repair of skeletal muscle injury.2.Zheng’s new injury medicine and ice compress have different advantages for the treatment of "damaged blood stasis syndrome" rats.Zheng’s new injury medicine is more conducive to the inhibition and regulation of IL series,and ice compress is more conducive to protecting the tissue structure.3.The mechanism of action of Zheng’s No.1 new injury drug and ice compress on the treatment of "damaged blood stasis syndrome" is different.Zheng’s new injury medicine may be through the inhibition of IL-1,TNF-α and IL-10,to achieve the effect of controlling inflammation and regulating immunity;ice pack may be through reducing bleeding and edema,reducing inflammatory mediators Release,at the same time down-regulate the expression of TNF-α,inhibit cell apoptosis,and achieve the effect of protecting tissue morphology and structure. |
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