Mechanism Explore Of The Change Of Cell Apoptosis Decided By TNF System On Injury Blood Stasis Syndrome

Purpose:Project intends to observe the effect of injury blood stasis syndrome on the apoptosis of skeletal muscle cells, from injury blood stasis rats serum TNF system changes. The project finds the specific mechanism of dynamic factors from TNF system related to the apoptosis process, in order to determine apoptosis effect on the progress and repair of traumatic injury with blood stasis syndrome, and to observe the correlation between the variation of apoptosis of skeletal muscle cells and rats serum TNF system changes.Method:80 rats were divided into 8 groups:Normal group,1H control group, model group,12h model group, 1D model group,3D model group,5D model group,7d model group, and 14d model group. These 8 groups are:without injury in rats and injury in 1 hour, injury in 12 hours, injury in 1 day, injury in 3 days, injury in 5 days, injury in 7 days, and 14 days. They were observed the effect on the apoptosis of skeletal muscle cells by the TNF family related indicators of TNF and TNF beta injury, Fas, sTRAIL, rank, RANKL, to reveal the pathological mechanism of skeletal muscle cell apoptosis.Results:Result 1:The characteristics of different time points in skeletal muscle of injury and blood stasis syndrome.There were 3 periods,2 turning points and 5 time characteristics. This is consistent with the pathological changes of pathological section and macroscopic feature. Such as 1 days of memory in the red hot pain, indicating that the immune cytokines fully mobilize, and then form a mechanism to promote apoptosis. And the damage of 1-7 days, damage clinical symptoms and pathological section gradually improved,7 days a variety of main symptoms and pathological repair and part of patients have persistent unhealed phenomenon.(1) 0-24 hours:early apoptosis. There was no significant abnormality in the level of apoptosis.(2) 1 days:two time point of apoptosis. The index of apoptosis reached a significant level, and the trend of tissue failure was formed.(3) 1-7 days of the body:stable phase of apoptosis. Apoptosis was maintained at a relatively stable level.(4) 7 days:the two apoptosis turning point in the repair of injury. Abnormal changes in the level of apoptosis,7 days there is an abnormal increase in apoptosis, this occurs only in the moment of behavior(5) after 7 days:the duration of apoptosis.7 days later, apoptosis is still high, and the level of apoptosis is still high, and the potential physiological and pathological injury of the body should be further studied after clinical and pathological symptoms completely disappeared.Result 2:TNF family(1) 1H:after the injury, TNF pathway, FAS pathway, RANKL immediately activated, that damage can be, TNF family members began to affect the body of blood stasis syndrome.(2) 12H:in the 24 hours of the prelude phase of apoptosis, TNF pathway, FAS pathway is highly open, which indicates that the two may be related to the 24 hours of apoptosis, is the prelude of the.(3) a day:RANKL activated again, and TNF pathway and Fas pathways restore calm, although apoptosis through TNF pathway or Fas pathway, but real finally starting point may and the other a failure mechanism of RANKL, indicating that the secondary injury may be a key RANKL failure mechanism itself.(4) 7 days:the two apoptosis turning point in the repair of injury. The attenuation of TRAIL and RANK may be closely related to the two day of apoptosis and clinical edema, and this phenomenon may be closely related to the repair effect of the negative threshold value of damage index.(5) for 14 days later:RANKL continued to decline, seat of tumor necrosis factor (TNF) broken bone composition, or for the destruction of substantially attenuated cellular components, which means may repair the gradually increasing, thus breaking cell physiology under within the scope of the slow decay may be a key factor in the blood stasis syndrome of skeletal muscle cells to repair damage.Conclusion:(1) The injury and repair of the blood stasis syndrome are caused by apoptosis, which including three stages and two activation points.(2) There was no significant abnormality in the level of early apoptosis suggest that it’s inappropriate using activating blood and resolving stasis method in early stage.(3) The two periods and one time piont of the skeletal muscle of injury and blood stasis syndrome are coincident with the weekly rhythm of chronomedicine.(4) The core of the acceleration and repair of the blood stasis syndrome of is the change of the TNF family. TNF pathway and FAS pathway are the prelude pathway of apoptosis induced by injury and blood stasis. RANKL pathway is the core of apoptosis of blood stasis syndrome, and is the key index of tissue damage and repair.(5) This project presents new three stages Syndrome Differentiation:early stage, which injuryed in 0-24 hours, should be treated by clearing or cold method; medium stage, which injuryed in 1-7 days, should be treated by activating blood and resolving stasis method; later stage, which injuryed in 7-14 days, should be treated by activating blood and resolving stasis method and tonifying method.(6) the syndrome differentiation of injury and blood stasis has the necessity of further research.