| purpose: By observing the effect of Fuling pill on the cartilage tissue morphology and the inhibitory effects of matrix metalloproteinases MMp-3,MMp-13 in synovial fluid on the knee osteoarthritis(KOA)model of Japanese white rabbits The change in the expression level of the agent TIMp-1 further deepens the study and understanding of the pathological process of KOA,and then explores the mechanism of Fuling pill Modified and Subtracted Formula in the treatment of KOA,providing a theoretical basis for the prevention and treatment of KOA in clinical practice.Material and method:Twenty-eight Japanese big-eared white rabbits were randomly divided into four groups: the normal group,the model group,the experimental group(that is,the Fuling pill Addition and Subtraction group),and the control drug group(that is,the diclofenac sodium group).Treatment,the remaining three groups of 21 Japanese big-eared white rabbits were taken to the left hind knee joint with the modified Hulth method for surgical modeling,one week after modeling,each group randomly selected a Japanese big-eared white rabbit to evaluate the modeling situation,the evaluation showed After successful surgical modeling,drug intervention was performed in strict accordance with the experimental design.The normal group and the model group were orally administered with 5 ml / d of distilled water;the experimental group was orally administered with 5 ml / d of conventional decoction of 10.22 g;the control group was dissolved with 10.4 mg of diclofenac sodium in 5 ml / d of distilled water.Stomach;Japanese big-eared white rabbits in each group were continuously administered by gavage for 4 weeks.During the treatment,the behavioral changes of the Japanese big-eared white rabbits in each group were observed.The local operation was observed and recorded.For processing.After 4 weeks of intervention treatment,the Japanese big eared white rabbits were sacrificed by air embolization,and the hematoxylin-eosin staining(HE)staining method was used to observe the synovial membrane and cartilage tissue morphology with light microscopy;Japan was extracted The knee joint fluid of big eared white rabbits was tested for the levels of MMp-3,MMp-13,and TIMp-1 by ELISA.Results: 1.Observing and scoring the knee cartilage tissue of Japanese big-eared white rabbits: The OARSI score of the model group was higher than that of the normal group,p < 0.05,and the data was statistically significant.The scores of Fuling pill addition and subtraction formula group and diclofenac sodium group were lower than the model group,p < 0.05,which was statistically significant.There was no statistical significance in the comparison of Fuling Wan addition and subtraction formula group with diclofenac sodium group,p> 0.05.2.The levels of MMp-3,MMp-13 and TIMp-1 in the knee joint fluid of Japanese big ear white rabbits in the model group,Fuling pill addition and subtraction formula group and diclofenac sodium group were significantly increased compared with the blank control group,and the difference was statistically significant(p < 0.01);the levels of MMp-3,MMp-13 and TIMp-1 in the knee joint fluid of Japanese big ear white rabbits in the normal group,Fuling pill group and diclofenac sodium group were significantly lower than those in the model group,and the differences were statistically significant.Significance(p < 0.01).The levels of MMp-3,MMp-13 and TIMp-1 in the knee joint fluid of the Japanese big ear white rabbit knee joint plus and minus decoction were slightly lower than those of the diclofenac sodium group,and the difference was not statistically significant(p> 0.05).There was no statistical difference in MMp-3 / TIMp-1 and MMp-13 / TIMp-1 between the groups(p> 0.05).Conclusion: 1.This experiment uses the improved Hulth method to prepare the KOA model of the Japanese big eared white rabbit.2.Fuling pills can significantly reduce the content of MMp-3,MMp-13 and TIMp-1 in the joint fluid of Japanese large-eared white rabbit KOA model,so as to treat Japanese large-eared white rabbit KOA model The decrease in the levels of these three indicators may be one of the mechanisms for the treatment of KOA by the addition and subtraction of Mi Fu Ling Wan..3.The results of gross observation suggest that the addition and subtraction of Mi Fu Ling Wan can improve the symptoms of KOA in Japanese white rabbits to a certain extent,and has protective effects on articular cartilage. |
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