Association Between RANK Gene Methylation And Osteoporosis In Elderly Men In Xinjiang

Objective:1.To understand the prevalence of osteoporosis in the elderly in Xinjiang’s agricultural and pastoral areas.2.Identify the levels of RANK gene methylation in elderly men in the agricultural and pastoral areas of Xinjiang.3.To reveal the internal connection between RANK gene methylation and osteoporosis in the elderly men of Xinjiang’s agricultural and pastoral areas.Methods:In April 2018,a three-stage random sampling method was used to conduct an epidemiological survey of elderly people aged 60 and above in Xinjiang’s agricultural and pastoral areas.A total of 2000 people were included in the study,of which 1920 had informed consent and participated in the study,and the response rate was 96.0%.After excluding unqualified study subjects according to the exclusion criteria,a total of 1846 cases(818 males and 1028 females)were included in the study.To analyze the prevalence of osteoporosis in the elderly population in agricultural and pastoral areas of Xinjiang.90 elderly males were randomly selected for a case-control study,and the extraction DNA,used heavy sulfite sequencing to identify meaningful methylation Cp G sites DNA methylation levels.To analyze the association between DNA methylation level of RANK gene and osteoporosis in elderly men in Xinjiang.Results:1.The prevalence of osteoporosis in the elderly population in Xinjiang’s agricultural and pastoral areas is 64.5%,of which the prevalence of osteoporosis in men and women is 46.5% and 78.9%;the prevalence of osteoporosis in women is higher than that in men(P< 0.05);the prevalence of osteoporosis in agricultural and pastoral areas in northern Xinjiang is higher than that in agricultural and pastoral areas in southern Xinjiang,which are 68.3% and 61.5%,respectively(P<0.05).2.The prevalence of osteoporosis is different between different age groups.As the age increases,the prevalence of osteoporosis generally increases.The prevalence of osteoporosis was highest in the age group of 80 years and older at 61.1%,followed by the 75 to 79 group at 51.6%;women were 75 to The 79-year-old group had the highest prevalence of osteoporosis at 93.9%,followed by the 70-74 group at 86.7%.3.The prevalence of osteoporosis in the elderly population in the agricultural and pastoral areas in the southern and northern Xinjiang was 61.5% and 68.3%,respectively(P<0.05).4.The results of Logistic regression analysis after correcting smoking,drinking and other factors suggested that female,age increase increased the risk of osteoporosis,and the risk of osteoporosis decreased with the increase of 25(OH)D(P<0.05).5.The methylation rate of RANK genes in the control group was higher than that in osteoporosis group among the elderly men in Xinjiang’s agricultural and pastoral areas(P<0.05).6.Using covariance analysis,after correcting the confounding factors,the methylation rate of RANK genes in the control group was higher than that of osteoporosis group in the elderly men in Xinjiang’s agricultural and pastoral areas.Conclusion:1.The prevalence of osteoporosis in the elderly population in Xinjiang’s agricultural and pastoral areas is higher in women than in men.The prevalence of osteoporosis in the elderly population in the northern Xinjiang agricultural and pastoral areas is higher than that in the southern Xinjiang agricultural and pastoral areas.2.Age and women are risk factors for osteoporosis,and 25-hydroxyvitamin D is a protective factor for osteoporosis.5.Among the elderly men in Xinjiang’s agricultural and pastoral areas,the reduction of methylation rate of RANK genes may be related to osteoporosis.