| Objectives To evaluate the treatment of Ginseng Baihu Decoction(GBH)for type 1 diabetic rats on islet immune injury via the model of type 1 diabetes mellitus(Type1 diabetes mellitus,T1DM)modeled by Streptozotocin(STZ),providing a better solution for further treatment of children with type 1 diabetes.Methods Feed 55 young rats for seven days,10 rats selected randomly were set to normal group(the normal control groups,NC group).The remaining 45 rats were injected with STZ 50mg/kg to establish the model of type 1 diabetes.Among of the 45 rats,40 rats were randomly divided into diabetic model group(the model control group,MC group),insulin group(the insulin group,INS group),Ginseng Baihu Decoction high dose group(the high dose group of ginseng Baihu Decoction,GBHH group),low dose group(the low dose group of ginseng Baihu Decoction,GBHL group),each group includes 10 rats.Each one rat in Insulin group was injected with insulin 2U/(kg·d),GBHH were given 12mL/(kg·d)decoction and GBHL were given 6mL/(kg·d)decoction by gavage,the normal group and model group were given intragastric of 2mL physiological saline,qd for 6 weeks.Recorded regularly for food,water,exercise capacity,urine volume;measurement of body weight(BW),random blood glucose(RBG);determinate the interferon gamma(IFN-gamma)and interleukin 4(IL-4)in serum by enzyme linked immunosorbent assay(ELISA)after killing rats to observe the condition of immune injury and repair.Observed the expression of chemokines via detecting stromal cell derived factor-1(SDF-1)and CXC chemokine receptor 4(CXCR4).compare the pathological changes in different groups.Results 1 The model of T1 DM young rats was successfully modeled by STZ: RBG > 16.7mmol/L,polypodiation,polyuria,and body weight loss.A few young rats died and blood glucose were not standard,so they were discarded.2 Before treatment,the diet and water volume of the experimental group were significantly higher than those of the NC group(P<0.01).After dosing,the diet and drinking water of GBH group decreased significantly,and dose related.It indicated that GBH can improve the symptoms of polydipsia and polyphagia in T1 DM rats.3 Before dosing,the BW in the experimental group was significantly lower than that in the NC group(P<0.01),and the RBG increased(P<0.01).After dosing,the BW of INS,GBHH and GBHL groups were higher than the MC group(P<0.05),and RBG decreased(P<0.05).4 After treatment,compared with group NC,IFN-gamma in group MC increased(P< 0.001)and IL-4 decreased(P<0.05).Compared with group MC,IFN-gamma decreased(P<0.01)and IL-4 increased(P<0.05).It shows that GBH can significantly reduce IFN-gamma,increase IL-4,and enhance the immunity of islets.5 After treatment,compared with group NC,the level of SDF-1 and CXCR4 in MC group decreased,and the difference was statistically significant(P< 0.001).Compared with the MC group,the SDF-1 of young rats in group INS increased(P<0.01)and CXCR4 increased(P<0.01);SDF-1 in young rats in GBHH group increased(P<0.001)and CXCR4 increased significantly(P<0.001).The SDF-1 in group GBHL increased(P<0.05)and CXCR4 increased(P<0.05).It shows that GBH can significantly increase the level of SDF-1 and CXCR4,so as to prevent and treat T1 DM complications.6 The Pancreatic histopathology showed that the pancreatic islet beta cells in group MC,INS,GBHH and GBHL were damaged more seriously than the pancreatic tissue in group NC,but the MC was significantly reduced,especially in the GBHH group.It shows that GBH can repair damaged pancreatic beta cells.Conclusions 1 GBH can significantly improve the clinical symptoms of T1 DM,such as polydipsia,polyphagia and emaciation.2 GBH can reduce the blood glucose,enhance the immunity of islet,prevent the occurrence of T1 DM complications,improve the pathological structure of the pancreas,and then play a role in the repair of the immune injury of the islet of T1 DM rats. |
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