The Study Of Recombinant Human Interferon α1b Injection Effects On Cellular Immune Function Of Children With Viral Encephalitis

Objective:To investigate cellular immunity by observing the changes in T lymphocyte subsets of peripheral blood in children with viral encephalitis;To observe and analyze changes of cellular immune function of Children with viral encephalitis,after combined treatment by Recombinant Human interferonα1b injection and provide clinical reference basis for the treatment of viral encephalitis.Method : The subjects who were diagnosed as viral encephalitis in pediatrics of The Second Hospital of Hebei Medical University from December in 2015 to November in 2016 were selected in this study,comparing with healthy children in age and sex.Control group was 45 healthy cases with26 boys and 19 girls from 1 to 12 years old;The treatment group was 60 cases of viral encephalitis,which included 34 boys and 26 girls from 2 to 13 years old.The treatment group was divided into 2 groups by random number table method:A group(Interferon treatment group)was 30 patients from 3 to 3years old including 16 boys,14 girls,B group(normal treatment group)was 30 patients from 2 to 12 years old including 18 boys,12 girls.A group was provided by combined treatment with Recombinant Human interferon α1b injection and B group was provided by antiviral and Symptomatic treatment.It was collected 3 ml venous blood within 48 hours of admission(onset within5 days)and 1 week after treatment from each patient and healthy children were collected 3 ml venous blood in outpatient service to test T lymphocyte subsets(CD4+/CD8+,CD8+,CD4+,CD3+T cells).Spssl7.0 was used in statistical analyze by X ± S and all the date were studied with t test in intergroup.There is no significant difference in P<0.05.Results:1 There was different expression level of T lymphocyte subsets between the treatment group before therapy and control group.It was decreased obviously(P<0.05)by the levels of CD3+T cells,CD4+T cells,CD4+/CD8+and increased(P<0.05)by the levels of CD8+T cells in the treatment group before therapy comparing with the control group.2 It was increased obviously(P<0.05)by the levels of CD3+T cells,CD4+T cells,CD4+/CD8+ and decreased(P<0.05)by the levels of CD8+T cells after therapy in the Interferon treatment group comparing with prior treatment.It was higher obviously(P<0.05)by the levels of CD3+T cells,CD4+T cells after therapy in the normal treatment group comparing with prior treatment,While there was no statistical significance(P>0.05)in CD8+T cells,CD4+/CD8+ after therapy and prior treatment in the normal treatment group.3 It was undifferentiated(P>0.05)in the levels of CD3+T cells,CD4+T cells,CD8+T cells,CD4+/CD8+ between the Interferon and normal treatment group before treatment.It was increased significantly(P<0.05)by the levels of CD3+T cells,CD4+T cells,CD4+/CD8+ and decreased significantly(P<0.05)by the levels of CD8+T cells after therapy in the Interferon treatment group comparing with the normal treatment group.Conclusions:The changes of immune function of children with viral encephalitis by the declining levels of CD3+T cells,CD4+Tcells,CD4+/CD8+indicate that immune function was restrained.Immune function was improved after treatment and increased significantly after combined treatment by Recombinant Human interferon α1b injection and it was worth popularization and application in the clinical work.