The Correlation Between The Course Of Viral Encephalitisand The Level Of T Lymphocyte Subsets Of Children

PARTl The Disorder of T Lymphocyte Subsets of Children with Viral EncephalitisObjective:Studying the change of T lymphocyte subsets in peripheral blood of children with viral encephalitis.Method:30 healthy children were selected randomly from our hospital as control group, and 60 homochronous children with viral encephalitis and abnormal cerebrospinal fluid from our department as observation group. Percentages of T lymphocyte subsets (CD3、 CD4>、CD8) in peripheral blood were detected with flow cytometry, and the change was observed. Data were presented as mean ± SEM. Student’s t-test was applied to compare the levels of T lymphocyte subsets (CD3, CD4, CD8) between the control group and observation group. A p-value<0.05 indicates the difference was statistically significant, and P< 0.01 means obvious statistical significance.Result:the ratio and average of CD3, CD4 and CD4/CD8 of control group were higher than observation group (P<0.05), while CD8 of observation group was obviously higher than control group (P<0.05).Conclusion:the number and function of T lymphocytes and their subsets of children with viral encephalitis are often in a state of disorder.PART2 The Correlation between the Course of Viral Encephalitis and the Level of T Lymphocyte SubsetsObjective:Exploring the correlation between the course of viral encephalitis and T lymphocyte subsets’level by analyzing the change of T lymphocyte subsets’level of children with viral encephalitis, so as to guide clinical diagnosis and treatment, and predict the timing of reexamining cerebrospinal fluid.Methods:Children with viral encephalitis and abnormal cerebrospinal fluid from our department were randomly selected. After 2 weeks of symptomatic treatment of antiretroviral therapy, decreasing intracranial pressure, regulating cellular immunity function and lowering body temperature,50 patients whose cerebrospinal fluid had recovered to normal were assigned as control group and 50 others who had not recovered as observation group. The T lymphocyte subsets’levels of the two groups were compared according to their changes before and after the treatment.Percentages of T cell subsets (CD3, CD4, CD8) in peripheral blood were detected with flow cytometry, and the change of T cell subsets was observed. Data were presented as mean ± SEM. Student’s t-test was applied to compare the levels of T lymphocyte subsets (CD3, CD4, CD8) of the control group and observation group. A p-value<0.05 indicates the difference was statistically significant, and P< 0.01 means obvious statistical significance.Results:The ratio and average of CD3, CD4 and CD4/CD8 in control group were higher than observation group (P<0.05), while CD8 of observation group was obviously higher than control group (P<0.05).Conclusions:The number and function of T lymphocytes and their subsets of children with viral encephalitis were often in a state of disorder. The patients’condition was in consistence with change in T cell subsets. At acute stage, T lymphocyte level was of certain guiding significance on the timing of reexamination during viral encephalitis treatment, and on the reduction of Children’s pain and iatrogenic injuries.