| Dahlia is one of the Asteraceae in high ornamental value.Landscaping has become,aspects,cut flowers production,food industry,new energy and a wide range of applications such as medical research,has a high economic and social value.Cultivated virus will cause the body gradually accumulate in the dahlia.Dahlia resulted from widespread viral disease,resulting in degraded quality dahlia.Dahlia industry thus been seriously challenged.For this experiment.Dahlia explants sterilization,tissue culture proliferation,rooting,acclimatization as the main research content,the purpose is to explore a set of convenient and safe to operate,and can be efficiently replicated virus-free propagation system in order to dahlia in vitro conservation of resources and the rapid propagation of technical support,but also to lay the technical foundation for the establishment of detoxification and genetic transformation system.1.Using 0.1%HgCl2,4%sodium hypochlorite,15%H2O2 as sterilizing agent,in the treatment of different sterilizing agents and different sterilizing time,different Dahlia '???' breed explant of pollution rate,browning rate and survival rate.In order to explore the suitable sterilization method.2.MS as basic medium,explores the different concentrations of 6-BA and NAA ratio on Dahlia of'???' iduced regeneration and proliferation;and respectively,MS,1/2MS with different hormone ratio combining,comparing different hormones on '???'rooting;to explore the impact of the hardening process of tissue culture planting survival rate,and the influence of different substrates on tissue culture planting survival rate.3.'???','???','????','???','??','???' of tissue culture experiments to compare different materials Hormone Concentration dahlia varieties multiplication factor Impact.The results are as follows:1.The results showed that the axillary buds as explants,with 75%ethanol sterilization soak 30,disinfection 0.1%HgCl2 4min for proper sterilization method,contamination rate below 25%of the highest survival rate can reach 65%.Leaves as explants,the use of 75%ethanol sterilization soaked 30s after the use of 0.1%HgCl2 sterilization 3min sterilization effect is best,the pollution rate below 25%,the final survival rate of up to 68%.2.The results show that the blade is dahlia is most suitable for the induction and culture of explants,the most suitable induction medium is MS+6-BA1.0mg/L+NAA0.8 mg/L+ sucrose 30g/L+ agar 7.0g/L,the highest bud induction rate can reach 63%.The suitable proliferation medium of '???'was MS+6-BA2.0 mg/L+NAA0.2 mg/L+sucrose 30g/L+ agar 7.0g/L,the proliferation coefficient was 6.31.The suitable rooting medium was 1/2MS+NAA0.2mg/L+ sucrose 30g/L+ agar 7.0g/L,the rooting rate reached 87.4%after 30d,and the average number of rooting was 2.7.Hardening process has a great influence on the planting survival rate has not been lower than the hardening hardening after planting survival rate of 5%;more appropriate planting matrix is:peat + perlite 1:3 ratios and the best way,the survival rate can reach 96%.3.The results show that similar '???','???',the best hormone combination 6-BA3.0mg/L+ NAA0.2mg/L.'???'and '???'proliferation coefficient can be reached 6.31 and 5.88;'????','???' best hormone combination for 6-BA2.0 mg/L+0.2mg/L and multiplication coefficient reached 5.70 and 5.79;'??','???',best hormone ratio for 6-BA2.0mg/L+NAA0.5mg/L,multiplication coefficient respectively of 5.99 and 5.41... |
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