| Morus alba Linn.is a perennial dicotyledonous woody plant,its leaves are natural diets for Bombyx mori L..So getting leaves is the main aim to plant the trees.Meanwhile it is also traditional medicine plants in our country. So Morus alba Linno is important economic trees and also important potential basis for sustainable development of sericultural industry.Using winter mulberry buds of Yu 71-1 petiole,stem,female flower and male flower as materials,this study established the regeneration,provided reference of culture young plant in factory.The mainly results are:1.In this test we studied the infection of auxin 6-BA,NAA,2,4-D, lanthanon lanthanum to mulberry bud polarization,screened out the proper polarization culture medium MS+6-BA 1.2 mg/L+2,4-D0.2 mg/L.2.Optimized the subculture condition of seeding,the best multiplication efficiency was obtained in the treatment of 6-BA 2.0 mg/L+2,4-D 0.2 mg/L; The multiplication cycle for about 45 days,can both guarantee the quantity and the quality of the adventitious buds;the cane sugar could improve the multiplication coefficient significantly,and the suitable concentration range were 30-40g/L.3.Researched the factors of regeneration of mulberry:TDZ,NAA,6-BA, IAA,breed,age and leaf position by orthogonal analysis,the results showed that the best embryogenic callus induction culture medium is MS+TDZ 1.0 mg/L+NAA 0.2 mg/L;the adventitious shoot polarization medium is MS+ 6-BA 3.0 mg/L+IAA 0.1 mg/L;the callus initiation rate of using 15d testtube of plantlet is 93.9%and adventitious bud differentiation rate is 98.3%.4.We studied the rooting and transplanting technique of Mulberry.The results show that the best educate medium is1/2MS +NAA 0.2 mg/L to mulberry seeding rooting.Mulberry seeding easily root in vitro when mulberry seeding marinate 30 min in 100 mg/L NAA concentration. Mulberry seeding survival rate is the highest when the rooted seeding temper 4 days.Pure sand is advantaged to the rooted mulberry seeding survival.
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