| Objective:To explore the mechanism of VIP receptor and its downstream signaling factors in order to explore the deep mechanism of the treatment of anorexia with spleen, and then to guide the clinical treatment of the disease.Methods:Selected transport spleen appetizer compound as the spleen appetizer treatment drugs, with a blank, low, middle and high doses of the drug containing serum on human gastric antral smooth muscle cells, which low dose equal to the human equivalent dose. The expression of VIP receptor(VPAC2) and downstream signal factors of eNOS and p-enos, PKA is determined from the protein level by using a Western blot.; at the same time, the use of nitrate reductase method for the determination of intracellular no concentration change trend, analysis the intrinsic meaning of the data in each group and the differences.Results:Physiological state of the human gastric antral smooth muscle cells only by, high dose of drug containing serum and VPAC2, eNOS, p-eNOS protein levels decrease was statistically significant (P<0.05), and between the middle and high dose groups no significant difference.The PKA protein level expression between the groups was not statistically significant. Also except the blank group, the drug containing serum on human gastric antrum smooth muscle cells no concentration has decreased, and was statistically significant (P<0.05), however low, middle, high groups had no significant difference.Conclusion:The spleen appetizer medicine in cell experiment on gastrointestinal peptide signal transduction regulation is cascade.VIPR and downstream signal molecular expression of the change trend is consistent, after reaching the effective dose of the human body to play its pharmacological effect, although the pharmacological effect produced does not increase with the dose. The regulation of VIP in the spleen and spleen can be realized through the NO dependent pathway, which is not related to the cAMP dependent protein kinase pathway. |
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