The Research On The Material Basis Of Bai Zhu Fu Ling Tang That Regulate Vasoactive Intestinal Peptide (VIP) Of The Spleen Qi Deficient Rat

Objective:To research the chemical compositions or components extracted from Baizhufuling Tang that can regulate Vasoactive Intestinal Peptide (VIP) and its receptor on the Spleen—Qi deficient rat, study the enhancement effect owing to compatibility and synergy of the chemical ingredients or components.Methods:①By using the system solvent extraction from the Bai Zhu Fu Ling Tang we isolated active component, and by further using modern Chinese chemical plant chemical means for isolation and identification, we obtained the effective part of all the components.②By applying chromatographic technique we rsearched the source of atractylenolide III in the decoction of Bai Zhu Fu Ling Tang and the influence of Atractylodes, Poria different proportion to the content of atractylenolideⅢ.③By Using the rat model of Spleen-Qi deficiency by the method of relieving stagnation of Qi and bitter cold starve and full in abnormal, we researched the influence of different components extracted from Baizhufuling Tang and combinations of these component on the Spleen Qi deficient rat, compared the different groups effect on the VIP, VIP receptor and receptor-binding of the rats, thence determined the effective components.Result:①Baizhufuling total polysaecharide, total complex compounds and atractylenolide III were isolated and extracted from Bai Zhu Fu Ling Tang decoction.②It is shown that Bai Zhu Fu Ling Tang decoction is similar to Atractylodes Decoction in the intensity and number of chromatographic peak, is largely different from Poria Under the condition of 215nm wavelength, the intensity of chromatographic peak of Bai Zhu Fu Ling Tang decoction is largest, and the number of chromatographic peak is most. In Bai Zhu Fu Ling Tang decoction, the content of atractylenolide③decreases in proper sequence when the proportion of Atractylodes and Poria change as the sequence of 1:1,3:1,2:1,1:3,2:3,3:2 and 1:2.③Compared with model group, VIP in blood plasma of the high dosage of Baizhufuling total polysaecharide group, the high dosage total complex compounds group, the high dosage atractylenolideⅢgroup and the high dosage of compound synergy group, Baizhufuling group was reduced (P<0.05 or P<0.01), Compared with model group; VIP in colon tissue of the high dosage of total polysaecharide group, the high dosage atractylenolide III group and the high dosage of compound synergy group, the middle dosage of compound synergy group, Baizhufuling group was reduced (P<0.05) Compared with model group, vasoactive intestinal peptide receptor 1 (VIPR1) in colon tissue of the high dosage of Baizhufuling total polysaecharide group, the middle dosage atractylenolideⅢgroup and the middle dosage of compound synergy group, Baizhufuling group was reduced (P<0.05 or P<0.01), Compared with model group, vasoactive intestinal peptide receptor 1 (VIPR2) in colon tissue of the high dosage of Baizhufuling total polysaecharide group, the high dosage of atractylenolideⅢgroup, the high dosage of compound synergy group, and the middle dosage of compound synergy group, Baizhufuling group was reduced (P<0.05 or P<0.01); Compared with model group, VIP receptor-binding in colon tissue of the high dosage of compound synergy group and Baizhufuling group was reduced (P<0.05)Conclusion:①Baizhufuling total amylose, total complex compounds and atractylenolideⅢare the Material Basis of Bai Zhu Fu Ling Tang that lower VIP and VIP receptor of the Spleen Qi deficient rat.②Atractylodes is the main source of the components of Bai Zhu Fu Ling Tang decoction and atractylenolide III is one of the important ingredients.③In Bai Zhu Fu Ling Tang decoction, the content of atractylenolideⅢis influenced by the the proportion of Atractylodes and Poria, and it is highest when the proportion of Atractylodes and Poria is 1:1, is lowest when the proportion of Atractylodes and Poria is 1:2.