Expression Of FAK, JAK1-STAT3 In Cervical Lesion Tissues And The Relationship With HPV

Objective: To investigate the expression of FAK, JAK1, STAT3, and HPV infection in cervical intraepithelial neoplasias and cervical carcinoma tissues, and explore the relationship of them in the development of cervical erosion.Methods: 143 detinitely pathological diagnosis cases of cervical tissue specimens were collected in the First Affiliated Hosptial, Henan University of Science and Technology from 2013 to 2014. Among them, 30 cases One for uterine fibroid uterine line all of normal cervical tissues, 113 cases of cervical tissue(CINⅠ 28 cases, CINⅡ-Ⅲ 34 cases and cervical cancer tissues 51 cases) were removed. The HPV infection status of 143 patients with PCR-Reverse dotblot hybridization were checked before the operation; The expression of FAK, JAK1 and STAT3 in 143 cases of cervical tissue with were detected by immunohistochemical staining S-P method. The differences of FAK, JAK1 and STAT3 in different cervical lesions and cervical cancer, which were in different clinical and different pathological type tissues were compared, and the relationgship between FAK, JAK1 and STAT3 with HPV infection in cervical tissues were analyzed, respectively.Results:1. The expression of FAK, JAK1 and STAT3 in the reference group, CINⅠ, CINⅡ-Ⅲ, and cervical cancer group increased individually, and there was a significant difference between every two groups(P<0.05).2. In cervical cancer tissues, there is no prominent difference of FAK, JAK1 and STAT3 expression in cervical cancer tissues at different conditiongs age(age<60,age≥60)(P>0.05).3. At different pathologic grades(well differentiate group, moderately-low dirrerentiation group), and different clinical stages(FIGO 2009Ⅰ-Ⅱstaging, Ⅲ-Ⅳstaging), different lymph transfer condition(with ervical lymph node etastasis, without cervical lymph node etastasis), the FAK, JAK1 and STAT3 statistica results(P<0.05): the FAK, JAK1 and STAT3 expression higher in moderately-low differentiation group, moderately-high clinical group, and with ervical lymph node etastasis.4. The positive detection rate of HPV-DNA in the reference group, CINⅠ, CINⅡ-Ⅲ, and cervical cancer group increased individually, and there was a significant difference between every two groups(P<0.05), except CINⅠ and CINⅡ-Ⅲ groups, who has no prominent difference(P<0.05).5. The expression of FAK, JAK1 and STAT3 in cervical tissues was a positive correlation with HPV infection(P<0.01).Conclusions:1. FAK, JAK1 and STAT3 almost have no expression in normal cervical tissues, but the ratio of the positive results in CINⅠ, CINⅡ-Ⅲ, and cervical cancer groups increased individually.2. The expression of FAK, JAK1 and STAT3 have close relationgship between different pathologic grades cervical cancer tissues, dfferent clinical stages, ervical lymph node etastasis. The results show that they have relationship with the invasion and metastasis of cervical cancer.3. The positive detectiion ratio of HPV-DNA in CINⅠ, CINⅡ-Ⅲ and cervical cancer group were significantly higher than the reference group. The positive detection of HPV-DNA in cervical cancer group were significantly higher than CINⅠ and CINⅡ-Ⅲgroup, while this ratio was little difference between CINⅠ and CINⅡ-Ⅲ groups. The results showed that HPV infection, has the relationship with the occurrence of cervical lesions, and the process of cervical lesions.4. The expression of FAK, JAK1 and STAT3 in cervical tissues was a positive correlation with HPV infection.