Study On The Mechanism Of Lijie Capsule In Treating RA Based On JAK1/STAT3 Signal Pathway

Objective:To observe the effect of Lijie Capsule on general condition,joint inflammation degree,synovial pathological changes and JAK1 and STAT3 protein expression level in synovial tissue of collagen-induced arthritis(CIA)rats,and to explore the mechanism of Lijie Capsule in treating rheumatoid arthritis(RA)from JAK1/STAT3 signaling pathway.Methods:Thirty SPF Wistar male rats were randomly divided into three groups,with 10 rats in each group.Except the normal group,collagen-induced arthritis(CIA)rat models were induced in Lijie capsule group and model group.From the 15 th day after the model was established,the Lijie capsule group was given experimental liquid,the normal group and the model group were given sterile water as experimental control,and the rest were free to eat and drink water.Three groups of rats were killed after 28 days of continuous intragastric administration.Experimental index detection: After the establishment of CIA rat model,the weight of rats was weighed every week,the lower limb volume of rats was measured by water volume method,and the arthritis index of rats was scored.The weight change,toe joint swelling degree and arthritis index integral(AI)of rats were used as the evaluation criteria for curative effect.The pathological changes of ankle synovium in each group were observed under light microscope.The expression level of JAK1 and STAT3 protein in rat synovium was observed by Western blot.Results:(1)body weight: compared with the normal group,the weight of CIA group and lijie capsule group decreased after modeling(p < 0.05);Compared with CIA group,the weight of rats in Lijie capsule group increased after 2 weeks(P < 0.05);(2)Toe swelling degree: Compared with the normal group,the toe swelling degree of rats in each group increased after modeling(P < 0.05).Compared with CIA group,lijie capsule group was significantly relieved after 2 weeks(p < 0.05);(3)AI value: Compared with normal group,AI index of CIA group and Lijie capsule group was significantly different after modeling(P < 0.01).Compared with CIA group,AI value in Lijie capsule group decreased after 2 weeks administration(P < 0.05).(4)Synovial pathology: Compared with CIA group,Lijie capsule group inhibited the proliferation of synovial tissue and decreased inflammatory cells in different degrees after intervention.(5)Expression of JAK1 and STAT3 protein:Compared with normal group,the expression of JAK 1 and STAT3 protein in synovial tissue of CIA group was higher,and the difference was statistically significant(P < 0.05).Compared with CIA group,the expression of JAK1 and STAT3 protein in lijie capsule group was decreased(P<0.05),but compared with normal group,the expression of JAK1 and STAT3 protein in lijie capsule group was still increased(P<0.05).Conclusion:(1)Lijie capsule can increase the weight of CIA rats and improve the general situation of rats;(2)Lijie capsule can effectively alleviate the joint inflammation of CIA rats,reduce the toe swelling and arthritis index of mice,but it does not achieve the effect of complete elimination;(3)Lijie capsule can effectively inhibit the proliferation of synovial tissue and reduce inflammatory cells to varying degrees,but can not completely eliminate synovitis of joints;(4)Lijie capsule can reduce the expression level of JAK1 and STAT3 protein in synovium and regulate JAK1/STAT3 signaling pathway benignly,which may be one of the important molecular mechanisms for its anti-inflammatory effect.