The Effects Of Acupotomy Therapy On Cervical Spondylosis Of Vertebral Artery Type Of Rabbits’ Nitric Oxide And Endothelin

Objective:To investigate the mechanisms of acupotomy for cervical spondylosis of vertebral artery type (CSA) by observing the change of Muscle Tissue, NO and ET of rabbits in model which are treated by acupotomy。Method:40 healthy common grade New Zealand rabbits were randomly divided into the blank group and the model group。 The blank group was not involved in the modeling and treatment, and maintained the conventional feeding, Build module model was prepared by injection of sclerosing agent, model preparation end after examined by TCD group 2 New Zealand rabbits left vertebral artery and basilar artery mean blood flow velocity (Vm)、pulsatility index (Pi) and resistance index (Ri) was compared and modeling group rabbits were signs evaluation, make sure the model successfully。Will make a successful model of 15 rabbits were randomly divided into acupuncture group and electro-acupuncture group and model group, Acupotomy group of acupotomy intervention,5 day intervention 1 time,2 times for a course of treatment intervention were 2 courses; Electro-acupuncture group adopt acupuncture treatment, the treated once a day, every 20 minutes,10 times for a course of treatment, were treated for 2 courses; model group received intervention, only as Acupotomy group and electro-acupuncture group reference object; from the blank group 5 rabbits were randomly selected as Acupotomy group and electro-acupuncture group and model group reference object。Continuous standardization at the end of the treatment, the animals of group 4 were made die parts of muscle tissue section and ear vein blood was taken, by pathological HE staining method, muscle tissue morphological changes observed under light microscope respectively, and the application of nitrate reductase method in detection of serum NO content and radioimmunoassay was used to measure the content of plasma ET, differences between the groups were compared。Results:(1) the rabbit model of CSA can be successfully prepared by the injection of a hardening agent。(2) under the light microscope, the morphological and histological changes with the blank group compared to model group, the cardiac muscle cells of severe necrotic, necrotic myocytes disappeared, interstitial moderate mononuclear inflammatory cell infiltration and scar; moderate electro-acupuncture group, the cardiac muscle cell necrosis, interstitial mild inflammatory cell infiltration, with slight hyperplasia of fibre tissue; Acupotomy group muscle cells slight necrosis, interstitial were no significant inflammatory cell infiltration and acupotomy intervention sites appear filled with connective tissue, but there was no granulation tissue or scar tissue。Summary:Acupotomy intervention compared with electro acupuncture therapy can rapidly promote inflammatory cells disappear, benign and sustained improvement in lesion muscle tissue and prompted the to normal skeletal muscle tissue repair。(3) in the model group, the serum NO content was significantly lower than that of the blank group (P＜0.01), and plasma ET content was significantly higher than that of the blank group (P<0.01); Acupotomy group and electro acupuncture group, the serum NO content and model group was significantly higher than that (P<0.05), plasma ET content decreased significantly (P<0.05); needle knife group, the serum content of nitric oxide (no) and EA group compared with the changes were not significant (P>0.05), with no statistical significance, but plasma ET content decreased significantly (P< 0.05), there was statistically significant difference。Summary:needle acupuncture intervention and therapy can be improved by CSA rabbit serum NO content and also significantly decreased plasma ET Content in the treatment of CSA and acupotomy intervention in the treatment of CSA in the overall effect is better than acupuncture therapy。Conclusion:Needle knife therapy in the macro morphology level by promoting the dissipation of inflammatory cells, improve the repair of muscle tissue, easing the inflammatory lesion and scar tissue of vertebral artery oppression; at the micro biochemical perspective through improve the content of serum NO and decreased plasma ET Content and make the vertebral artery to restore to normal physiological relaxation state, to ensure the normal perfusion of brain tissue, so as to achieve the effect of CSA treatment.