The Effect And Mechanism Of Guizhi Fuling Pills On Controlling The Proliferation And Inducing The Cell Cycle Arrest Of The Human Breast Cancer Cell MCF-7

ObjectiveTo study the growth, cell cycle and the associated protein EGFR, Cyclin A2 and Cdk2 of the human breast cancer cell MCF-7 under the influence of Guizhi Fuling Pills,and this will give a theoretical foundation to the clinical application of the Guizhi Fuling Pills. MethodDetect the growth of human breast cancer cell MCF-7 under the different dose of Guizhi Fuling Pills by the method of MTT. Measure the cell cycle of human breast cancer cell MCF-7 by the method of flow cytometry. Detect the associated protein EGFR, Cyclin A2 and Cdk2 by the method of Western blot. Result1. Compared with the blank control group, the cell inhibition developed after 24 hs, 48 hs, 72 hs by using the different dose of the Guizhi Fuling Pills, and the OD values had a significance decline(P<0.01).2. The morphology of the human breast cancer cell MCF-7 changed after 48 hs of using the different dose of Guizhi Fuling Pills.3. The number of DNA synthesis stage cell increased after 48 hs of using 1800μg/m L, 2700μg/m L Guizhi Fuling Pills(P<0.01).4. After 48 hs of using the 1800μg/m L, 2700μg/m L Guizhi Fuling Pills, the associated protein Cdk2 had a decline(P<0.05), EGFR also had a decline(P<0.05). Compared with the blank group, protein Cyclin A2 didnot had a statistical significance in the concentration of 1800μg/m L(P>0.05), but in the concentration of 2700μg/m L, there is a decline expressed(P<0.05). Conclusion1. Guizhi Fuling Pills can control the proliferation of the human breast cancer cell MCF-7, which appeared time dose dependent. The morphology of the human breast cancer cell MCF-7 can also changed by the use of Guizhi Fuling Pills.2. Under a certain dose, Guizhi Fuling Pills can keep the human breast cancer cell MCF-7 at DNA synthesis stage after 48 hs.3. Guizhi Fuling Pills can control the proliferation of the human breast cancer cell MCF-7 by decline the expression level of the associate protein EGFR.Guizhi Fuling Pills can keep the human breast cancer cell MCF-7 at DNA synthesis stage by decline the expression level of the associate protein Cyclin A2 and Cdk2.