Liver Should Spring Correlation Between Allergy And Theoretical And Experimental Research

In TCM theory,"Correspondence between Man and Nature" which is an important theoretical basis In traditional Chinese Medicine means man and nature is an integrated whole."Liver correspondence to Spring" which is an important part of "Correspondence between Man and Universe"Refers to the liver and the spring climate change with synchronous oscillations and collaborative relationship.there is many similarities understanding between Chinese medicine for five organs system and the respiratory system, motor system, genitourinary system, digestive system, circulatory system of Western Medicine,Such as the wind evil of traditional Chinese Medicine and the allergen of modern medicine.The combination of traditional Chinese and Western medicine research internal control theory provides a good bond and bridge.The research group of "correspondence between man and nature" in the study, has been on the "kidney correspondence to winter","lung correspondence to autumn" and "heart correspondence to summer" has certain theoretical and experimental studies, but "liver correspondence to spring" is still belongs to blank.On the theory research of "liver correspondence to spring" contributes to deepening on the understanding of liver function in seasonal changes,is important clinical guidance to occur allergic disease prevention and treatment in the spring,and deepen the understanding of the essence of the liver.1ObjectiveThrough literature this study found that spring is the season of high incidence of allergic disease. we Conduct the four seasons change on allergy of immunology experimental study,begin with the relationship of wind evil and allergic disease.From the allergy of immunology in modern medicine perspective to explain the essence of the traditional Chinese medicine "liver correspondence to spring".2Methods2.1Theoretical methodsThis study which used the method of literature and logical analysis, to allergic diseases occur in the spring you as the clue, correlation of the wind evil of traditional Chinese medicine and the allergy of immunology in modern medicine as a starting point discuss liver and allergic of immune correlation.In order to find the modern medicine material basis of liver in traditional Chinese Medicine.2.2Experimental methods2.2.1Experimental animalsExperimental animals:twenty-four SD male rats, clean,weighted150-170g, brought forty days before vernal equinox, summer solstice, autumnal equinox and winter solstice in2010, were randomly divided into three groups:normal group, fake group and operation group (pinealectomy group), there were eight rats in every group. All rats received natural light, ate and drank freely, and were controlled under certain room temperature. Fake group and operation group are operated a month before vernal equinox, summer solstice, autumnal equinox and winter solstice. All the rats would be raised before being beheaded at8o’clock at night in vernal equinox, summer solstice, autumnal equinox and winter solstice.2.2.2Test methodsSerum immune response related to substance lgE, lgG, TNF-A.IL-13:using ELISA method.Serum Mel:using ELISA method. The thymus index, spleen index direct weighing.2.2.3Analysis method:All the data were showed by mean±standard error. The differences of groups were tested by SPSS11.0. The values were calculated with Duncan’s multiple range test or Student’s test. P<0.05meant obvious difference.3Results3.1Results of theoretical study (1)From research of "Liver correspondence to Spring" by history of Chinese medicine,it can be found that liver function will be appeared rhythmic changes in the spring,thus affecting the whole human body function. Deep understanding of liver function of rhythm characteristics have the profound significance for ome seasonal incidence of disease prevention and treatment.(2)Allergic diseases belongs to the category of Western medicine,but it has a rapid onset, rapid onset, easy to repeated attacks, parts move uncertain and changeable, itching intolerable characteristics,the same of Chinese medicine diseases caused by pathogenic wind.(3)The onset of allergic disorders is allergen invasion causes the allergic immune response process, while the majority of allergic diseases predominate in the allergic immune index IgE.4.Chinese medicine in clinical treatment of allergic diseases were found during treatment from liver and dealing with the wind has apparent curative effect.3.2Results of experiments3.2.1Influence on immune organs by seasonal variation and extracting pinealectomy(1)Thymus index:Physiological group had trend spring>summer>autumn>winter, there was a very significant difference (P<0.01) in autumn and winter compared with in spring and summer;In the same season,there was a significant difference (P<0.05) and showing the trend of content descending in spring and summer and rising in autumn and winter that operation group compared with physiology group.(2)Spleen index:Physiological group had the trend of content summer>spring>autumn>winter,there was a very significant difference (P<0.01) in spring and summer compared with in winter,there was a significant difference (P<0.05) in autumn compared with in winter;In the same season,there was no significant difference but showing the trend of content descending in spring and winter and rising in autumn and summer that operation group compared with physiology group.3.2.2Influence on immune factors in serum by seasonal variation and extracting pinealectomy(1) TNF-α in Serum:Physiological group had the trend of content winter>autumn>summer>spring, there was a very significant difference (P<0.01) in autumn and winter compared with in spring and summer;In the same season,there was no significant difference but showing the the trend of content rising that operation group compared with physiology group.Three groups of seasonal rhythm always embodied the trend winter>autumn>summer>spring,and the content was highest in winter compared with other season.(2)IL-13in Serum:Physiological group had the trend of content winter>summer>autumn>spring, there was a very significant difference (P<0.01) in spring and summer and autumn compared with in winter;In the same season,there was a very significant difference (P<0.01) in autumn and showing the the trend of content rising that operation group compared with physiology group.Although the seasonal rhythm of the content IL-13of pseudo operation group and operation group was changed,the content was highest in winter compared with other season.(3)IgG in Serum:Physiological group had the trend of content summer>spring>winter>autumn, there was a very significant difference (P<0.01) in spring and autumn and winter compared with in summer;ln the same season,there was a significant difference (P<0.05) in winter and showing the the trend of content rising that operation group compared with physiology group;Three groups of seasonal rhythm was always embodied in spring and summer higher than in autumn and winter.(4)IgE in Serum:Physiological group had the trend of content winter>spring>summer>autumn,there was a very significant difference (P<0.01) in summer and autumn compared with in spring,and was a very significant difference (P<0.01) in autumn and winter compared with in summer,and was a very significant difference (P<0.01) in autumn compared with in winter; In the same season,there was a very significant difference (P<0.01) in autumn and showing the the trend of content rising that operation group compared with physiology group;Three groups of seasonal rhythm was always embodied in spring and summer higher than in autumn and winter.3.2.2Influence on by seasonal variation and extracting pinealectomyMel in serum of Physiological group had the trend of content winter>spring>summer>autumn,there was a significant difference(P<0.05)in autumn compared with in spring,and the content in spring is highest than other season; In the same season,there was a significant difference (P<0.05) in spring and showing the the trend of content descending that operation group compared with physiology group;Operation group Mel in serum content did not differ significantly between seasons, and physiological group rhythm was significantly different.4Conclusions(1)According to "Literature review method" and "Logical analysis" and allergen belonging to the Chinese wind evil category,by spring high incidence of allergic disease as carrier,we can deduce that The modern medicine allergic immune belongs to the traditional Chinese medicine liver function category.(2)In clinical we can cure allergic diseases which is high incidence in spring. from the liver and the wind.(3)In the six index of allergy and Immunology,serum IgG and IgE, thymus and spleen index has increased in spring,so the four index are closely related with the allergic diseases which is high incidence in spring.TNF-a in Serum and IL-13in Serum are closely related with the allergic diseases which is high incidence in winter.(4)The pineal and Mel has a modulatory role on allergen immunotherapy,The specific performance is that the pineal have inhibition to serum immune factors, and have complex regulatory role to the immune organs.Mel has a complex regulatory role to the body’s immune system.(5)The connotation of "Liver correspondence to Spring" is related to the pineal and Mel and Allergic immune of body...