Wen Yang Zhen Shuai Granules(WYZS Granules) On Chronic Heart Failure Caused By Adriamycin Model Experimental Study Of Rabbits P38Expression Of Influence

Objective:Discuss Wen Yang Zhen Shuai granules(WYZS granules) on chronic congestive heart failure caused by adriamycin (chronic heart failure, CHF) model of rabbit heart function, expression of proteins of P38lightning (T-P38lightning) and P38lightning phosphorylation level of protein expression (P-P38lightning) P38lightning detection and quantitative analysis of the expression of mRNA transcription level, for Wen Yang Zhen Shuai particles clinical use to provide certain theoretical basis.Methods:60rabbits were randomly selected15for normal group, the remaining45were given Adriamycin by intravenous injection for4weeks. After4weeks, all rabbits were did echocardiography.13rabbits were selected for4weeks model group randomly, and we removed their hearts to detection and quantitative T-P38, P-P38and quantitative of P38-mRNA expression. Then the30rabbits which were given Adriamycin were assigned randomly into5groups by the heart function: chronic heart failure (CHF) model group, positive control group, Wen Yang low-dose group, Wen Yang middle-dose group, Wen Yang high-dose group. In addition to the normal group injected with saline, the other5groups were injected with Adriamycin to the8th week. On the5th week, the normal group and CHF model group were given saline, all of the Wen Yang groups were given Wen Yang Zhen Shuai granules(WYZS granules) infusion, the positive control group were given enalaprilat for4weeks. After experiment, all rabbits were did echocardiography and be removed their hearts to detection and quantitative T-P38, P-P38and quantitative of P38-mRNA expression.Results:1. Compared with normal group, the cardiac function in4weeks model group and CHF model group decreased significantly (P＜0.01), the cardiac function of positive control group and Wen Yang group were improved, compared with CHF model group, the difference was significant (P＜0.05). Compared with positive control group and Wen Yang low-dose group, the cardiac function of Wen Yang middle-dose and high-dose group was better(P＜0.05). The differences between positive control group and Wen Yang low-dose group, Wen Yang middle-dose and high-dose group were not significant (P＞0.05).2. The differences of T-P38among all groups were not significant (P＞0.05). Compared with normal group, P-P38of the other group were increased (P＜0.05). But positive control group and Wen Yang groups’ P-P38were decreased compared with CHF model group(P＜0.05). The differences between positive control group and Wen Yang high-dose group, Wen Yang low-dose and middle-dose group were not significant (P＞0.05). The differences of P38-mRNA among all groups were not significant (P＞0.05).Conclusion:Wen Yang Zhen Shuai granules (WYZS granules) can improve heart function on rabbit model of chronic heart failure induced by Adriamycin. And middle-dose and high-dose of Wen Yang Zhen Shuai granules (WYZS granules) were better than Wen Yang low-dose and enalaprilat. The effects of Wen Yang Zhen Shuai granules (WYZS granules) may realized by means of decreasing P-P38to regulate P38signal transduction pathway. But the specific mechanism needs further study.