The Study Of The Relationship Between The Seven Essential Elements In Serum And Some Chronic Diseases Of The Population Of Junan County

ObjectiveMineral elements play an important role in sustaining human life by participating in the body’s metabolism, physiology, biochemical reaction, energy transformation. The function of mineral elements is related to the content of elements in our body. At the certain range, the normal structure and function can be maintained. When the concentration of the elements is below or above the need of the body, the normal function of the body can be affected. It could cause the deficiency of the mineral elements, toxicities and even the death of our body. In this present study, we determined the contents of potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), copper (Cu), iron (Fe), and zinc (Zn) in serum of4210people in Junan. By this study, we want to understand:1The medical reference range of K, Na, Ca, Mg, Cu, Fe and Zn in the serum of the people.2The internal relationships between the contents of K, Na, Ca, Mg, Cu, Fe and Zn in serum and diabetes mellitus, hyperlipaemia, obesity, virus B hepatitis and some common women’s diseases(breast tumor, fibroid, menstrual colic).This study results could provide certain references and basis to study the relationship between the mineral elements concentration and the diseases, then to make reasonable dietary guideline.Methods1The choice of investigated objectsThe selected sample villages should be able to represent the background level of Junan in geographical environment, economic level and social environment, and the population turnover should be small and have a good compliance. The inhabitants in the sample towns were chosen as the investigation objects of this research.2Inquiry and investigationThe questionnaires were finished by each resident by face to face interview as a unit. The questionnaires included basic condition of family members, personal health condition monitoring table, chronic disease situations in half a year, married women (15～49years old) health questionnaire and so on(Attachment chart A-H)3Health examinationsThe heights, weight, waistline and the blood pressure of the selected people were measured, and then determined their fasting blood glucose, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol and hepatitis B in the serum.4Determination of the concentrations of K, Na, Ca, Mg, Cu, Fe and Zn in serumThe concentrations of K, Na, Ca, Mg, Cu, Fe and Zn in serum were by flame atomic absorption spectrophotometer.5The confirming of the medical reference range of K, Na, Ca, Mg, Cu, Fe and Zn concentrations in serumThe SPSS16.0statistical analysis software was used to get the medical reference range of the elements, and then the comparisons of the level of the seven essential elements between man and women were analyzed.6The study of the relationship between the content of K, Na, Ca, Mg, Cu, Fe and Zn in serum and some chronic diseasesThe investigated objects were separately divided into diabetes mellitus group, hyperlipidaemia group, virus B hepatitis group, obesity group, breast tumor group, fibroid group, menstrual colic group and their corresponding control groups. And then the SPSS16.0statistical software was used to discuss the relationship between the content of K, Na, Ca, Mg, Cu, Fe and Zn in serum and diseases.Results1The result of the choice of the investigation objects4210people were selected for the research from6sample villages in Junan followed the methods.2The methodological study of the content of K, Na, Ca, Mg, Cu, Fe and Zn in serum with flame atomic absorption spectrophotometer2.1K:a good linear response was obtained over the range of0.2-4mg/L with correlation coefficient0.9992, the standard curve was Y=0.0685X+0.0064,the detection limits was0.147mg/L, the inter-day RSD were2.11%,1.45%,1.22%, the intra-day RSD were5.31%,4.66%,6.21%, the recoveries were98.0%,97.2%,101.0%.2.2Na:a good linear response was obtained over the range of1～5mg/L with correlation coefficient0.9987, the standard curve was Y=0.1851X+0.0229,the detection limits was0.055mg/L, the inter-day RSD were2.98%,2.21%,1.87%, the intra-day RSD were2.56%,3.82%,4.28%, the recoveries were97.3%,101.8%,101.0%.2.3Ca:a good linear response was obtained over the range of0.5～10mg/L with correlation coefficient0.9998, the standard curve was Y=0.0219X+0.0024,the detection limits was0.181mg/L, the inter-day RSD were2.57%,1.81%,2.09%, the intra-day RSD were3.53%,4.27%,5.32%, the recoveries were96.7%,98.2%,94.4%。2.4Mg:a good linear response was obtained over the range of0.05～1mg/L with correlation coefficient0.9998, the standard curve was Y=0.2176X+0.0021,the detection limits was0.025mg/L, the inter-day RSD were2.43%,2.09%,1.67%, the intra-day RSD were4.56%,5.10%,5.73%, the recoveries were100.6%,106.7%,98.2%.2.5Cu:a good linear response was obtained over the range of0.1～1.6mg/L with correlation coefficient0.9996, the standard curve was Y=0.0203X+0.0002,the detection limits was0.021mg/L, the inter-day RSD were0.99%,1.21%,0.79%, the intra-day RSD were2.61%,2.45%,2.26%, the recoveries were103.3%,98.3%,100.5%.2.6Fe:a good linear response was obtained over the range of0.05～1mg/L with correlation coefficient0.9995, the standard curve was Y=0.0339X+0.0006,the detection limits was0.032mg/L, the inter-day RSD were1.32%,0.98%,0.57%, the intra-day RSD were2.83%,2.46%,2.61%, the recoveries were93.3%,103.3%,98.2%.2.7Zn:a good linear response was obtained over the range of0.02～0.4mg/L with correlation coefficient0.9976, the standard curve was Y=0.1740X+0.0024,the detection limits was0.015mg/L, the inter-day RSD were1.68%,1.42%,0.99%, the intra-day RSD were1.95%,2.36%,2.28%, the recoveries were98.6%,93.3%,102.2%.3The medical reference range of K, Na, Ca, Mg, Cu, Fe and Zn concentrations in serumThe medical reference range of K, Na, Ca, Mg, Cu, Fe and Zn concentrations in serum were83.44～296.94mg/L,2.00～3.67g/L,23.27～284.10mg/L,8.48～35.06mg/L,0.38～1.49mg/L,0.81～4.21mg/L and0.34～3.66mg/L in male,82.60～312.00mg/L,2.29～3.72g/L,26.94～308.29mg/L,8.52～35.83mg/L,0.42～1.59mg/L,0.77～3.98mg/L and0.33～4.36mg/L in female.Compared with male, the contents of Ca in female was significantly higher (P<0.05).The Fe concentration in male was higher than female (P<0.01). There are not significantly differences of K, Na, Mg, Cu, Zn in male and female (P>0.05).4The relationship between the contents of K, Na, Ca, Mg, Cu, Fe and Zn in serum and diabetes mellitusThe K, Mg contents were significantly lower than the control group (P<0.05), and the contents of Cu, Fe and Cu/Zn were significantly higher than the control group (P<0.05).The contents of Zn, Cu were positive correlated to the blood sugar. Ca and Mg were the protective factors for people against diabetes mellitus.5The relationship between the contents of K, Na, Ca, Mg, Cu, Fe and Zn in serum and hyperlipaemiaThe contents of Fe and Zn in serum and Zn/Cu in the hyperlipidaemia group were higher than the control group (P<0.01), and the K, Mg and Cu concentrations were significantly lower (P<0.05). The contents of Mg, Cu in serum and TG were negatively correlated, the contents of Fe and Zn in serum and TG were positively correlated (P<0.01). The content of Cu in serum was negatively correlated to TC (P<0.01). The contents of Na, Mg and Cu in serum were positively correlated to HDL-C (P<0.01). The contents of K, Na and Fe in serum and LDL-C were positively correlated. The content of Cu in serum was negatively correlated to LDL-C (P<0.01).6The relationship between the contents of K, Na, Ca, Mg, Cu, Fe and Zn in serum and virus B hepatitisThere were not significantly differences of the contents of K, Na, Ca, Mg, Cu, Fe and Zn in the virus B hepatitis from the control group(P>0.05). The Mg, Zn contents were the protective factors of the virus B hepatitis.7The relationship between the contents of K, Na, Ca, Mg, Cu, Fe and Zn in serum and obesityThe contents of K, Na and Zn in serum of the obesity group was significantly lower than those in the control group (P<0.05). The contents of K, Ca, Mg and Zn in serum of obese men were lower than the contents in obese women. The contents of K, Na, Cu and Zn in serum were obvious negative correlated to the BMI level.8The relationship between the contents of K, Na, Ca, Mg, Cu, Fe and Zn in serum and some common women’s diseases (breast tumor, fibroid, menstrual colic)The Fe content of fibroid group was significantly lower than the control group (P<0.05). The content of Zn of breast tumor group was lower than the control group (P<0.05). The Ca and Mg levels of the menstrual colic group were significantly lower than the control group (P<0.05).Conclusions1The FLA A method for the detection of K, Na, Ca, Mg, Cu, Fe and Zn in serum was simple, quick, accurate, repeated well and the sample dosage was fewer.2The contents of Fe, Ca in serum were different between man and women in Junan County. The Fe serum level in male was higher than female, and the Ca level in female serum was higher than male. There were not significantly different of K, Na, Mg, Cu and Zn between man and women.3The diabetes mellitus patients were lack of K, Mg, so K and Mg should be supplied for the treat of the diabetes mellitus patients. We should focus on the contents of Ca and Mg during the diagnosis and the treat of diabetes mellitus.4The hyperlipaemia patients were lack of K, Mg and Cu, so the hyperlipaemia patients should be supplied K, Mg and Cu in the common life.5The contents of Mg and Zn were the protective factor of virus B hepatitis, so the virus B hepatitis were suggested to be supplied Mg and Zn for the prevention of the virus B hepatitis.6The obesity people were lack of K, Na and Zn, so K, Na and Zn should be supplied for the reducing of obesity.7Enough contents of Fe were helpful for the cure of fibroid. And supplying the content of Zn could be used to the cure and prevention of the breast tumor. It was useful for the prevention of menstrual colic and relief of the pain to provide enough Ca and Mg.