Study On The Expression Of Gastric Mucosal Interferon-γ And Interleukin-12in The Splenogastric Hygropyrexia Syndrome Belonging To H.Pylori-associated Gastropathy

Objective:By detecting Helicobacter pylori infection、pathological changes of the Gastric mucosa、Interferon-γ、Interleukin-12expressions of the patients with the Splenogastric Hygropyrexia belonging to H. pylori-associated Gastropathy, and deficiency of spleen-QI Syndrome, to explore the deep relations of the pathophysiology of Splenogastric Hygropyrexia Syndrome, Helicobacter pylori infection and IFN-γ,IL-12from the local Angle, This is from the Angle of modern medicine interpretation of the nature of Splenogastric Hygropyrexia Syndrome.Method:1. Choose114cases with CSG and PU including83cases belonging to Splenogastric Hygropyrexia Syndrome(among them,12cases belong to the group of Splenogastric Hygropyrexia Syndrome Hp(+) and heavy dampness impairing heat,15cases belong to the group of Splenogastric Hygropyrexia Syndrome Hp(+) and simultaneous onset of dampness and heat,17cases belong to the group of Splenogastric Hygropyrexia Syndrome Hp(+) and heavy heat impairing dampness,39cases belong to the group of Splenogastric Hygropyrexia Syndrome Hp(-)) and19cases belong to the group of Splenic deficiency Syndrome(among them,8cases belong to the group of the deficiency of spleen-QI Syndrome HP(+),11cases belong to the group of the deficiency of spleen-QI Syndrome HP(-).12cases of recruitment of healthy voluteers as normal control group. Subjects in each group were asked to complete the clinical observation of form and carried out the assessment of symptoms. 2. we collected two pieces of the tunica mucosa sinus ventriculi under the gastroscope. One of them used the fast urease test, the other one was placed in neutral buffered formalin and embedded by the paraffin.3. Hp was detected by toluidine blue staining method and fast urease test.The inflammation of musosa was observed by common hematocylin-eosin staining method.4. Take the two step of ElivisionTM plus immunoristochemical labeling method to detect the expression of gastric mucosal Interferon-γ and Interleukin-12.Result:1. Analysis of common materials:There were no obvious difference for the groups on the sex, age, the course of disease and the rate of Hp(p>0.05). So the groups could be compared by each other.2. The comparison of the Hp infective rate and the Hp infection level:The comparison showed that the Hp infective rate of the Splenogastric Hygropyrexia Syndrome groups were slightly higher than the Splenic deficiency Syndrome groups. But there were no obvious difference of the Hp infective rate and the Hp infection level between the the Splenogastric Hygropyrexia Syndrome groups and the Splenic deficiency Syndrome groups (P>0.05)3. The ralationship of the main clinical symption soreThe main clinical symption sore between the the Splenogastric Hygropyrexia Syndrome groups and the Splenic deficiency Syndrome group (P>0.05) were similar.4. The comparison of the gastric mucosa inflammation level and the gastric mucosa inflammation activity in each group and the relationship with Hp infection.No matter the Gastric mucosa inflammation leve or the gastric mucosa inflammation activity, Splenogastric Hygropyrexia Syndrome group are severer than deficiency spleen QI Syndrome group,and both of the above are significantly severer than the normal control group, on the whole, the result have the tendency that Splenogastric Hygropyrexia Syndrome group>deficiency spleen-QI Syndrome group; the Gastric mucosa inflammation leveland the gastric mucosa inflammation activity of the Splenogastric Hygropyrexia Syndrome group and deficiency spleen-QI Syndrome group are severer than the same type of Hp(-),the ralationship among the Hp infection and the Gastric mucosa inflammation leveland the gastric mucosa inflammation activity (P<0.001)5. The expression of gastric mucosal interferon-γand interleukin-12in each group.Interferon-γmainly Positioning in cytolymph, of the membranae propria and gland cell. Interleukin-12mainly Positioning in cell nucleus of the membranae propria, epithelium and gland cell, but some of the Interleukin-12Positioning in cytolymph which with inintestinal metaplasia or Atypical hyperplasia.6. The expression of gastric mucosal interferon-γand interleukin-12In each group is that Splenogastric Hygropyrexia Syndrome group and deficiency spleen-QI Syndrome group are significantly higher than the normal control group. There have the tendency that Splenogastri Hygropyrexia Syndrome group>deficiency spleen-QI Syndrome group.7. The comparison of the three sub-divided groups of Splenogastric Hygropyrexia Syndrome Hp(+),have the tendency that simultaneous onset of dampness and heat group>heavy dampness impairing heat group>heavy heat impairing dampness group, and the expression of Interleukin-12of the three sub-divided groups are higher than the normal control group. So, there have the tendency that simultaneous onset of dampness and heat group>heavy dampness impairing heat group>heavy heat impairing dampness group.6. The ralationship of the Hp infection and the expression of interferon-γ and interleukin-12.Compare with the Hp(-) group, the expression of interferon-γ and interleukin-12are both higher than the Hp(+) group, there were obvious difference of them.Conclusion:1.Hp infection play a role in the pathogenic process of Splenogastric Hygropyrexia Syndrome. Hp infection result in the pathological changes of the Gastric mucosa.2. There may exist the relativity to some extent between the expression of gastric mucosal interferon-γand interleukin12and Splenogastric Hygropyrexia Syndrome. The high expression of ginterferon-γand interleukin-12may reflect the connotation that dampness and heat is excessive and Quarrel with each other, but the upright qi is not deficiency.3. The expression of interferon-γ and interleukin-12of the three sub-divided groups of Splenogastric Hygropyrexia Syndrome, has different tendency. The dampness and heat formate the Splenogastric Hygropyrexia Syndrome and and to help forward the expression of interferon-γ and interleukin-12. The tendency shows that, while the dampness and heat combine with each other the pathological changes of the Gastric mucosa may be more serious.4. There may exist the relativity to some extent between the Hp infectionand the expression of interleukin-12, but not the interferon-γ, Hp infection may be played a role of inducing in the expression of interleukin-12.