Study On The Relationship Between Splenogastric Hygropyrexia Syndrome Of ROU And The Effects Of Zn²⁺, Immunologic Function And HP Infection And Influence On KouKuiFang

Recurrent oral ulcer (ROU),refered as recurrent aphthous ulcer (RAU),is a limitations and isolated oral mucous membraneous ulcer which posses the feature that recurrent attacks,difficulty on healing,continuous attacks,lasting long time from years to decades.A lot ofstudy and research on etiopathogenisis of ROU had been reported,but there are notconclusive etiological factor about ROU.It has been presumed that origin of ROU wereinduced by composite factors such as genetic gene,immunization,infection,microelement,mental state and so on,and immunization is one of the most important factors in thepathogenesis of ROU.Splenogastric Hygropyrexia Syndrome is the common syndrome in the clinicaldiagnosis of ROU and we have explored and studied the nature of SplenogastricHygropyrexia syndrome.Professor LAO Shaoxian from the Guangzhou traditional Chinesemedicine university has researched the type of syndrome in clinical diagnosis of ROUthoroughly and summarized that Splenogastric Hygropyrexia Syndrome is the commonsyndrome by many years clinical experience.So combination XHS and HPXLT are appliedfor clinical treatment of ROU.In this paper,to explore the role of Splenogastric Hygropyrexia Syndrome in clinicalROU,Splenogastric Hygropyrexia Syndrome in ROU were analyzed,the herbal medicinewere applied,and therapeutic efficacy of QingReHuaShiFa were evaluated for therapeuticmechanism research and foundational theory exploration of ROU.Literature ResearchROU are called as 'recurrent aphtha'.By retrospective analysis of ancient pertinentliterature about recurrent aphtha,there are four period:establishing,sprouting,forming andconsummating in the theory of Splenogastric Hygropyrexia Syndrome.And the nature of Splenogastric Hygropyrexia Syndrome were studied by epidemiology,morphology anddynamics of gastroenteropathy,HP infection,cellular energy metabolism,water channelprotein,gene regulation and so on.The type of syndrome in ROU had been explored from different point of view andfrom different administrative level by scholars.These researches had integrated thepathogenesis of ROU in abstracto and improved the clinical treatment of ROU in practice.However,there are fewer studies on aggregate analysis of ROU pathogenesis.Clinical and Empirical StudyClinical StudyObjective:To study the effects of Zn²⁺,immunologic function and HP infection on SplenogastricHygropyrexia Syndrome in ROU and influence of KQF.Investigate Object:34 disease group of patient suffered with ROU,from the First Affiliated Hospital,Guangzhou Traditional Chinese Medicine University,male 19,female 15,with mean age38.41±10.85yrs,differentiated the symptoms and signs as Splenogastric HygropyrexiaSyndrome,and 10 normal control group,from healthy volunteer without ROU,male 6,female 4,with mean age 39±10.59 yrs.Methods:1.All the Investigate Objects received clinical survey by special investigators thoughquestionnaire method (shown in attached table),including the spots of ulcer,the area ofulcer,intermission time of ulcer,index of pain,and so on.2.The patient suffered with ROU and differentiated the symptoms and signs asSplenogastric Hygropyrexia Syndrome were treated with KQF for 2 weeks.The serum Zn²⁺,CD₃,CD₄,CD₈,CDVCD₈,IgG,IgA,IgM were detected respectively before and after thetreatment.And the normal control group received the placebo treatment and detected thesame parameters.3.Data is presented as means±SD for number of samples animals.Data were analyzed byStudent's t-tests for unpaired data and by Genrral Linear Model for multi-class datd.P<0.05 was considered statistically significant,enumeration data were analyzed bychi-square criterion.All statistical analyses were performed with the statistics softwareSPSS 11.5for Windows.Results:1.Compared with normal control group,the patientof ROU differentiated as Splenogastric Hygropyrexia Syndrome had a increase of IgA (P<0.01)and a decrease of CD₃,CD₄ andCD₄/CD₈ (P<0.05)significantly.2.the linear correlation and regression between serum contents of Zn²⁺ and CD₃,CD₄,CD₈,CD₄/CD₈,IgG,IgA,IgM both normal control group and ROU with SplenogastricHygropyrexia Syndrome group were analyzed,and the results showed that the ROU withSplenogastric Hygropyrexia Syndrome group (had)a change on serum contents Zn²⁺ and humoral and cell immune function,and there (were)not obvious associativity in theseparaneters.3.There were a strong correlation between serum Zn,CD₃,CD₄,CD₄/CD₈,IgA and ROU.Low CD₄/CD₈ and high IgA are the risk factor of ROU,and high level of serum Zn²⁺ and CD₄ were protective factor of ROU.4.there were not exist of HP in the oral ulcer surface and saliva from ROU patients by aHP-DNA detection.5.The mechanism of KQF on Splenogastric Hygropyrexia Syndrome of ROU were toincrease the serum zinc,CD₃,CD₄,CD₄/CD₈ ratio (P<0.01)and to decrease the IgA (P<0.01.In clinic,KQF could improve the clinical symptom,extend the intermission time of ulcer of ROU disease,decrease the spots and area of ulcer,and relief the pain of ulcer(P<0.05).Empirical StudyObjective:To study the relation between oral ulcer and serum zinc,immune function and discussthe effect of KQF on ROU in animal model.Animals:76 SD rats,with mean weight 180±20g,purchased from the Laboratory Animal Center,Guangzhou traditional Chinese medicine University,were maintained under specificpathogen-free conditions.Methods:1.The animals were divided into seven groups:A.normal control group,B.oral ulcermodel group,C.zinc prevention and cure group,D.zinc and low dose KQF group,E.zincand high dose KQF group,F.KQF prevention and cure group and G.composite factorsmodel with KQF group.2.the external appearance,mental status and excrement were oberved,and the oral ulcerstate were detected.Before and after the treatment of zinc or KQF,the serum level of zinc,CD₃,CD₄,CD₈,CD₄/CD₈,IgG,IgA,IgM were measured.The samples from oral ulcer tissue were collected for light microscope and electron microscope detection.3.Data is presented as means±SD for number of samples animals.P<0.05 was consideredstatistically significant.All statistical analyses were performed with the statistics softwareSPSS 11.5 for Windows.Results:1.Before being made oral ulcer model,compared to that in group A,the serum indices,including blood serum zincum concentration,CD₄,CD₄/CD₈,were increased significantlyin group C (P<0.05),and so did in group D and group E;Compared group C to that ingroup A,no significant difference was noted as to IgA,IgG,IgM,and CD₈,respectively,andso did in group D and group E.2.After being made oral ulcer model,compared to that in group A,the serum increase ofIgA,CD₈ and a decrease,including blood serum zincum concentration,CD₄,CD₄/CD₈,were increased significantly in group B(P<0.05),and so did in group F and group G.Compared to themslf before being made oral ulcer model,the serum increase of IgA,CD₈and a decrease,including blood serum zincum concentration,CD₄,were increasedsignificantly in group C(P<0.05),and so did in group D and group E.3.After the treatment:compared to that in group A,the serum increase of IgA,CD₈ and adecrease,including blood serum zincum concentration,CD₄,CD₄/CD₈,were increased significantly in group C(P<0.05),and so did in group D and group E.Compared to themslfbefore being made oral ulcer model,the serum increase of IgA,CD₈ and a decrease,including blood serum zincum concentration,CD₄,were increased significantly in groupC(P<0.05),and so did in group D and group E.Conclusions1.There are a strong correlation between serum Zn,CD₃,CD₄,CD₄/CD₈,IgA and ROU.Low CD₄/CD₈ and high IgA are the risk factor of ROU,and high level of Zn and CD₄ areprotective factor of ROU.2.There are a decrease of serum zinc,CD₄,CD₄/CD₈ ratio,IgM and a increase of CDs,IgA in the ulcer period of ROU.The results showed that the Splenogastric HygropyrexiaSyndrome have the variation of serum zinc and immune function.But there are not obviouscorrelation between these parameters,and showed that they are maybe independent riskfactor of ROU.3.There are maybe a indirect correlation between HP infection and ROU.4.The treatment of zinc can increase the CD₄ and CD₄/CD₈ ratio,affect the immunefunction of ROU for the clincial treatment of ROU. 5.The mechanism of KQF on Splenogastric Hygropyrexia Syndrome of ROU were toincrease the serum zinc,CD₃,CD₄,CD₄/CD₈ ratio and to decrease the IgA.In clinic,KQFcan improve the clinical symptom,extend the plastochrone of ROU disease,decrease thespots and area of ulcer,and relieaf the pain of ulcer.6.The animal model established by composite factors have a concordance to clinicalsymptom of ROU,which have the appearance of anorexia,moving slowly,lazing,as wellas dental ulcer with a slow recover.7.Combination Chinese medicinal herb and zinc provide ROU a preferable therapeuticefficacy,for Chinese medicinal herb only improve the immune function and theinterference of zinc only affect the metabolism of serum zinc.8.These are not significant difference between large and low dose KQF in the therapeuticefficacy.