| Prescription is a tool with a specific function and the main treatment for disease prevention, Which is made by a number of herbs compatibility. There is a a high degree of correlation or focused references between the compatibility their primary disease pathogenesis or pathological evidence. This is called "The correlation between formula and syndrome."Evidence for a specific disease may have a few formulas work, but the results are different, or the same role in different disease and syndrome prescriptions would be different treatment. "The correlation between formula and syndrome" is the core principle of traditional Chinese medicine dialectical thinking, but also to learn prescription based on the logic of justification. Study the relationship between the formula and the syndrome to reveal the syndrome differentiation theory has important theoretical and practical significance.this study in rats with chronic astricting+diet+excess fatigue to replicate animal model of liver depression and spleen deficiency.To act as entry points from the appearance of animal model to compare the observations of rats'the appearance of behavioral change on the role of the differences and similarities, with the Liver, Spleen, Liver Spleen different effects of chaihushugansan. Sijunzitang and chaishusijuntang.And then and the ascertain the central neurotransmitters and biochemical changes of the model animal and and the changes result in the different formulae.This dissertation contains of literature review and experimental research. Research evolutions of clinic and experiment about the model of stagnation of liver and deficiency of spleen (GYPX), chaihushugansan. Sijunzitang and chaishusijuntang are summed up.Experimental study observed stagnation and spleen deficiency syndrome in rats characterized by the appearance of behavior and central neurotransmitters and related biochemical changes in things and chaihushugansan. Sijunzitang and chaishusijuntang. relevant role of the models.Methods:Rats were randomly divided into normal control group, liver depression and spleen deficiency syndrome group (hereafter referred to as the model group), chaishusijuntang groups,SiJunzi tang group and chaihushugansan group. Each group has 12 annimals. the latter 4 groups of rats were placed in restraint devices every day 8:00 am restriction of 3 h,2:00 pm place filled with warm water (22±1℃) swimming in a large plastic bucket 10 min, every other day feeding (every other day ban food, enough to eat every other day) for 4 weeks; normal control group did not exert any stimulating natural feeding. At 15 days of modeling, the rats were treated with traditional Chinese medicine Chaishusijun Tang 4.27g/kg, Sijuzitang 4.53g/kg, ChauhuShugan San 4.2g/kg orally,1 time per day; model group with the control group were given distilled water for 14 days. And in the first 7d,14d,21d,28d, points early, middle and later time points the rats were observed and recorded changes in the behavior of state, activity level, emotional reactions, skin hair, food intake, sleep, stool state, etc.; In the first 7 days a week 2:00-2:30 pm Determination of body weight of rats in each group; in the first 14d,28d by open field test rats were recorded within 5min crawling cell number, number of rearing, modification time and Time on the central grid.8:00 am on the 29th day were decapitated, rapidly stripping the Ice hippocampus, hypothalamus and brain stem, placed in liquid nitrogen tanks stored, tested. High Performance Liquid Chromatography and Fluorescence Detection hippocampus Asp, Glu, GABA, Gly, Tau; measured by radioimmunoassay in the hypothalamus of cAMP, PGE2; by enzyme-linked immunosorbent assay in the brain stem P450 enzyme activity and content.Statistical Methods:The data were to described an x±S, After the single factor analysis of variance (one-way ANOVA) to test, the more groups were analyzed using Student Newman Keuls Test test. All data using SPSS 13.0 for windows statistical processing software.Results:1. Syndrome Model of rats and related changes in appearance characterized the role of herbs:1 week after modeling, model group, chaishusijuntang group, SiJunzitang group, chaihushugansan group's behavioral activity, emotional reactions, skin hair, diet, sleep and defecate state all did not change. Model rats 2 weeks after modeling showed reduced activity, reduced the level of activity, mood irritability, food intake, fur ring true Chak, easy to wake up, then dry, etc.; 3 weeks after modeling,the model group began to slow down the movement, emotions stability, food intake, fur is not Chak, tired of lying, loose stools; 4 weeks after modeling, the significant reduction of activities, get together or even tired of lying to sleep, emotional state from the beginning of the irritability to depression, the struggle to reduce, food intake, coat dry mess, loose stool and so on. Compared with model group, the rats in the administration of Chinese medicine after 1 week (3 weeks after modeling), activity, mood stabilization, food has increased, fur, sleep and bowel movements were seen in varying degrees of improvement in the state. After 2 weeks of administration (4 weeks of modeling), chaishusijuntang group significantly increased behavioral activity, responsive, smooth fur close to the whole, food increased, the depth of sleep, moderate, formed stool;sijuzitang grope, the rats'fur, food, sleep and improvement of stool condition obviously; rats of chaihushugansan in behavioral state, activity level, emotional response and so on were see significant improvement.2. Syndrome Model of body weight change and related prescriptions in Action: During the experiment, body weight of the normal rats was significantly increased,while the body weight in model group and three treated group gained weight more slowly, in which the model group, chaishusijuntang Group and sijunzitang group body weight had a negative growth in the first weekend. The weigh of the model group rats, chauhushugansan group showed a downward trend,after 4 weeks.Compared with normal group, the body weight of model group and chaishusijuntang group, SiJunzitang group, chaihushugansan group were significantly decreased at the end of 1w,2w,3w,4w. the difference was significant (P <0.05). Compared with model group, three treated group no significant changes in body weight difference (P> 0.05); and three dose groups no significant difference in body weight change (P> 0.05).3. Syndrome Model of autonomous behavior in rats and related changes in the role of herbs:Compared with normal group, he number of crawling cells of model group and the three Chinese medicine groups significantly increased (P<0.01 or P <0.05) in the first 14 days of modeling, the difference; The rats of model group and chaishusijuntang vertical had increased, the difference was significant (P<0.05); model group, sijunzitang group modification time and the central grid residence time were extended, of which modifiede time'difference was significant (P<0.05). After 2 weeks of administration (At the 28th day), compared with the normal group, the number of rats crawling cells increased model group, sijunzitang group and chaishusijuntang group, the differences were significant (P<0.05), the number of rats crawling cells of chaishusijuntang close to the normal level (P> 0.05); The model group and sijunzitang group vertical increased in the number, the difference was significant (P<0.05), chaishusijuzitang group and chaihushugans group didn't increased obviously (P> 0.05); The modification time and time of he central cell residence of model group and three Chinese medicine group, were extended, but the difference was not significant (P> 0.05). Compared with model group, crawling cell number and were s number of rearing of 3 Chinese medicine groups significantly lower. the number of crawling cells of chaishusijuntang group reduced significantly, the difference was significant (P<0.05).4. Syndrome Model of central nervous and related biochemical changes in the role of prescriptions:(1) Compared with with the normal group, hippocampus Asp (aspartic acid) of model group content tended to increase Glu (glutamic acid), GABA (y-aminobutyric acid), Gly (glycine), Tau (taurine) were decreased, but the differences were not significant (P> 0.05); PGE2 and cAMP in the hypothalamus changed not significantly different (P> 0.05); brainstem P450 enzyme activity and enzyme content in no significant difference (P> 0.05). (2) Compared with model group, three Chinese medicine group had no significant change in the above index (P> 0.05). Conclusions:The chronic restraint+diet+excess fatigue Disloyalty prepared Syndrome Model of rats showed reduced activities, get together to sleep, depression, fighting less, eating less, fur dry mess, loose stool, weight loss and so on, accompanied by the enhanced spontaneous activity behavior.2 weeks after the treat with chaishusijuntang, sijunzitang, chaihushugansan, rats can characterize the appearance of varying degrees of improvements and self-regulate certain activities.Then intervention effct of chaihusijuntang group is best,chauhushugansan group and sijuzitang has had play respective role in the behavioral state,emotional reactions and the skin hair, sleep and so on. These results show that the model is similar to the stagnation and spleen deficiency syndromes in some features, chaishusijuntang and this model has a higher correlation, consistent with "Shuganjianpi corresponding spleen deficiency and stagnation" in TCM experience.This study Syndrome Model of Syndrome Evaluation study provides some ideas for evaluating TCM Syndrome.lt also can provided some of modern basis for understanding the meaning"evidence related parties" and function of the relevant formulas. |
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