Shugan - Spleen - Shuganjianpi Side, Liver Stagnation And Spleen Deficiency Syndrome

"Correlation between Formula and Syndrome" is the core content of in the diagnosis and treatment based on differentiation of symptoms and signs and formula-ology. The function and usage of formula not only havecorrelation with medical compatibility but also compatible with the syndrome which it will have effect on, that is the main effect of the formula depends on a considerable extent on the pathology and the role of targets of medicalcompatibility. Although the progress has been made about "Correlation between Formula and Syndrome",less scientific evidence is provided for it. Based on the background, the research is began with different formulae's action on the same model.The scientific meaning of Correlation between Formula and Syndrome is revealed through comparing and observing the effect of three formulae's action on the same model.The dissertation was to explore the rats'syndrome model of stagnation of live anddeficiency of spleen (GYPX) which was made by the method of compound causes of diseaseaccording to the TCM's theory of attack, clinical manifestation and some physiological and pathological characteristics of some modern diseases about this syndrome and to acquire the Chinese syndrome and some biological characteristics of this model through observing manytargets about the nerve-endocrine-immunity system; On these achievements, we observed and compared similarities and differences of action of, SJZT and CSSJT which had therespective function of soothing liver, invigorating spleen andsoothing liver and invigorating spleen on GYPX model and probed into the associatedcharacter and partial modern biological basis of these associate between the effectiveness ofthe three prescriptions and the model of disease and syndrome on the condition of different prescription on the same syndrome. Finally, we explored genes expression of the model and CSSJT influencing it by applying microarray technology, and deeply approachedmolecular mechanism of the model and cssjt influencing it.This thesis is divided into two main parts:literature review and experimental research. literature review include clinnic and experimental research of GYPX, CHSGS, SJZT and CSSJT. The change of immune system, HPTA, HPA, digest system and blood circulation made by CHSGS, SJZT and CSSJT and Gene expression profiles associated with preliminary exploration of the syndromes of GYPX deficiency of rat model and study of the effect of CSSJT on the model. Research 1:the change of immune system of the model of GYPX syndrome and the effect of CHSGS, SJZT and CSSJT on the model of GYPX syndromeMethods:The rats were assigned to five groups randomly:normal group, model group of stagnation of liver and deficiency of spleen, CHSGS group, SJZT group and CSSJT group,12 rats in each group. The rats of latter four groups were administrated by chronic restricting and excess fatigue with out constant diet for four weeks and normal group rats were fed as usual.The rats of three medical groups were orally administrated with CHSGS, SJZT, CSSJT (lml·100g-1·d-1) respectively from the 15th day to the end and rats of normal and model groups were given equal volume of distilled water. Interleukin-2 (IL-2) and interleukin-6(IL-6) in serum and spleen T lymphocyte proliferation rate of groups were observed by the method of RIA.Results:IL-2, IL-6, spleen T lymphocyte proliferation rate of model group decreased significantly. IL-2 of CHSGS,SJZT,CSSJT increased significa-ntly. IL-6 of CHSGS, SJZT, CSSJT increased in varying degrees, and CHSGS and CSSJT were ignificant. Spleen T lymphocyte proliferation rate of CHS-GS, SJZT, CSSJT increased in varying degrees,and CSSJT was significant. Conclusion:The abnormal changes turned up in immune system. CSSJTwas better than CHSGS and SJZT in adjusting these abnormal changes.Research 2:the change of throid system of the model of GYPX syndrome and the effect of CHSGS, SJZT and CSSJT on the model of GYPX syndromeMethods:The rats were assigned to five groups randomly:normal group, model group of stagnation of liver and deficiency of spleen, CHSGS group, SJZT group and CSSJT group,12 rats in each group. The rats of latter four groups were administrated by chronic restricting and excess fatigue with out constant diet for four weeks and normal group rats were fed as usual.The rats of three medical groups were orally administrated with CHSGS, SJZT, CSSJT (lml·100g-1·d-1) respectively from the 15th day to the end and rats of normal and model groups were given equal volume of distilled water. T3, T4, TRH and TSH of groups were determined by the method of RIA.Results:T3,T4,TRH and TSH of model group were significantly decreased. T3 and T4 in serum of CHSGS, SJZT and CSSJT groups were significantly increased. TRH and TSH in serum of CSSJT group markedly increased.Conclusion:The abnormal changes turned up in thyroid system. CSSJT was better than CHSGS and SJZT in adjusting these abnormal changes.Research 3:the change of HPA of the model of GYPX syndrome and the effect of CHSGS, SJZT and CSSJT on the model of GYPX syndromeMethods:The rats were assigned to five groups randomly:normal group, model group of stagnation of liver and deficiency of spleen, CHSGS group, SJZT group and CSSJT group,12 rats in each group. The rats of latter four groups were administrated by chronic restricting and excess fatigue with out constant diet for four weeks and normal group rats were fed as usual. The rats of three medical groups were orally administrated with CHSGS, SJZT, CSSJT (1ml·100g-1·d-1) respectively from the 15th day to the end and rats of normal and model groups were given equal volume of distilled water. CRH, ACTH, COR, CRH in the hypothalamus were determined.by the method of RIA.Results:CRH,COR in plasma and CRH in the hypothalamus of model group increased significantly and ACTH in plasma of model group decreased significantly. CRH in plasma of CHSGS, SJZT, CSSJT decreased in varying degrees,not significantly. ACTH in plasma of CHSGS, SJZT, CSSJT increased significantly. COR in plasma of CHSGS, SJZT, CSSJT decreased in varying degrees,and chsjt was significantly. CRH in the hypothalamus of CHSGS, SJZT, CSSJT decreased in varying degrees,and CSSJT and chsgs were sig-nificantly.Conclusion:The abnormal changes turned up in HPA. CSSJT was better than CHSGS and SJZT in adjusting these abnormal changes.Research 4:the change of digest system of the model of GYPX syndrome and the effect of CHSGS, SJZT and CSSJT on the model of GYPX syndromeMethods:The rats were assigned to five groups randomly:normal group, model group of stagnation of liver and deficiency of spleen, CHSGS group, SJZT group and CSSJT group,12 rats in each group. The rats of latter four groups were administrated by chronic restricting and excess fatigue with out constant diet for four weeks and normal group rats were fed as usual.The rats of three medical groups were orally administrated with CHSGS, SJZT, CSSJT (1ml·100g-1·d-1) respectively from the 15th day to the end and rats of normal and model groups were given equal volume of distilled water. MTL and SS in plasma by the method of RIA, D-xyloseexcretion rate in urine by the method of adjacent aminotoluene, pepsin by the method of spectrophotometer,and SDH and G-6-PD in liver by the method of NBT were determined.Results:MTL and SS in plasma, pepsin, and SDH and G-6-PD in liver of model group increased significantly. D-xyloseexcretion rate in urine of model group decreased significantly. MTL in plasma of CHSGS, SJZT, CSSJT group decreased significantly. SS in plasma of CHSGS and CSSJT group decreased in varying degrees,and SJZT group increased significantly. D-xyloseexc-retion rate in urine of CHSGS, SJZT, CSSJT group increased in varying degrees, and SJZT and CSSJT were significant. Pepsin of CHSGS, SJZT, CSSJT group decreased in varying degrees, and CSSJT was significant. SDH in liver of CHSGS, SJZT, CSSJT group decreased significantly. G-6-PD in liver of CHS-GS, SJZT, CSSJT group increased significantly,and CSSJT was significant. Conclusion:The abnormal changes turned up in digest system and SDH and G-6-PD in liver. CSSJTwas better than CHSGS and SJZT in adjusting these abnormal changes.Research 5:the change of blood circulation in liver of the model of GYPX syndrome and the effect of CHSGS, SJZT and CSSJT on the model of GYPX syndromeMethods:The rats were assigned to five groups randomly:normal group, model group of stagnation of liver and deficiency of spleen, CHSGS group, SJZT group and CSSJT group,12 rats in each group. The rats of latter four groups were administrated by chronic restricting and excess fatigue with out constant diet for four weeks and normal group rats were fed as usual.The rats of three medical groups were orally administrated with CHSGS, SJZT, CSSJT (1ml·100g-1·d-1) respectively from the 15th day to the end and rats of normal and model groups were given equal volume of distilled water. Hemorheology measured by blood viscosimeter, blood routine test by automatic blood cell counter, blood coagulation function by Automatic coagulation analyzer were determined.Results:The index of erythrocyte aggregation decreased, hematocrit increased significantly and the index of erythrocyte deformability had no change. The whole blood viscosity under different shear rates of model group's rats increased significantly and reduced viscosity had no change. Blood routine test and blood coagulation function had no change. The index of erythrocyte aggregation, hematocrit, index of erythrocyte deformabi-lity, reduced viscosity under different shear rates, blood routine test (WBC,RBC,HGB,PLT) and blood coagulation (APTT,PT,PTA,Fib) of CHSGS,SJZT,CSSJT group had no change. The whole blood viscosity under different shear rates of SJZT group decreased significantly. The whole blood viscosity under higher shear rates of CHSGS group decreased sign-ificantly.Conclusion:The abnormal changes turned up in blood circulation. CSSJTwas better than CHSGS and SJZT in adjusting these abnormal changes. Research 6:Exploration of gene expression on the syndromes of GYPX and the effects of cssjt.Methods:The rats were assigned to 3 groups randomly:normal group, model group of stagnation of liver and deficiency of spleen, CSSJT group,12 rats in each group. The rats of CSSJT group were administrated by chronic restricting and excess fatigue with out constant diet for four weeks and normal group rats were fed as usual. The rats of CSSJT group were orally administrated with CSSJT (lml·100g-1·d-1) respectively from the 15th day to the end and rats of normal and model groups were given equal volume of distilled water. We extract RNA from liver, and tag RNA by fluores-cence, hybridizate and cleanse, scan chip, collect picture and analyze data; At last we inquest functions of obstained different genes by gene bank of http://www. genome.ad. jp/kegg/.Results:There are 59 differentially expressed genes in model group, genes, among them,27 genes are upregulation and 32 downregulation, referring to inquest known functional genes as followed:Scdl,Dbp,Upp2, Slc28a2,Sds,Hpn,Gldc,Tm7sf2,Gcat,Btg3,Lin7a,Zfp354a,Rgs2,Duspll,Ldha; CSSJT group has 25 differentially expressed genes, among them 13 are upregulation and 12 aredownregulation; referring to inquest known functional genes as followed:Hhex,Myc,Hmgcr,Ccl7,Bubl.Conclusion:The model of the syndromes of GYPX exists abnormal expression of part genes, known functional genes of involved in metabolism of glucose and metabolism of fatty; CSSJT has generally regulate to genes expression of GYPX, the main known functional genes mainly influence metabolism of glucose and cell circle. The results cue that abnormal expression of several genes is possibly molecular foundation of the syndromes of GYPX, however related formula of treating the syndromes of GYPX have complicate regulate to many genes including abnormal expression genes of the synd-romes of GYPX.The change of the function of stomach and intestine, NEI network, blood circulation, SDH and G-6-PD in liver had been observed in GYPX model made bythe method of chronic astricting, excess fatigue and out of constant diet on ratsBased on the result, we observed the different action of CHSGS, SJZT, and CSSJT on the GYPX model made by the method of chronic astricting, excess fatigue and out of constant diet on rats and discovered that the function of CSSJT was better than that of CHSGS and SJZT in the way of adjusting abnormal change of immune system, HPTA, HPA,digest system and blood circulation. The syudy had provided objective evidence for cor-relation between prescription and syndrome.