Jiawei Dangguibuxue Tang Has An Impact On The Expression Of Renal Tissue In Rats Of Hyperlipidemia Ctgf Protein And Mrna

Jiawei Dangguibuxue Tang Has an Impact on the Expression of Renal Tissue in Rats of Hyperlipidemia CTGF Protein and mRNAObjective:From the Jiawei Dangguibuxue Tang in hyperlipidemic rats with renal connective tissue growth factor (CTGF) protein and mRNA expression, explore its mechanism of action of anti-renal injury.Methods:With the Modified Dangguibuxue Tang pairs of high-fat diet for renal injury model of intervention, rats were randomly divided into normal group, model group, impastation group, flavored Dangguibuxue Tang low, middle and high-dose treatment group. The treatment groups after 12 weeks were administered continuously, using semi-quantitative immunohistochemical detection of CTGF protein expression, fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) detection of the expression level of CTGF mRNA. From the various groups of CTGF protein and mRNA expression levels explore hyperlipidemia-induced renal damage and Jiawei Dangguibuxue Tang its mechanism of action.Results:Immunohistochemistry results showed that the model group CTGF protein expression in renal tissue were significantly higher than the normal group (P<0.01), the treated rats the expression of CTGF protein in varying degrees of reduced,especially to Jiawei Dangguibuxue Tang high-dose group of the most obvious. Followed by the positive control group, the dose of Chinese medicine, Chinese medicine low dose group. Fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) tests showed that the model group kidney CTGF mRNA expression was significantly higher than the normal group (P<0.01), the treated rats CTGF mRNA expression of varying degrees of reduced, especially to Jiawei Dangguibuxue Tang high-dose group of the most obvious. Followed by the positive control group, the middle-dose group, the low-ose group.Conclusions:Jiawei Dangguibuxue Tang on Hyperlipidemia prevention and treatment of renal injury in rats may be related to reduced CTGF protein and mRNA expression.