| Medicinal fungi is a class of fungi which are possessed of medicinal value and could cure diseases.Versicolor is a kind of wood-rotting medicinal fungi. The active ingredient PSK of Versicolor not only have significantly curative effect on the chronic hepatitis and hepatitis B, but also could prevent cancers and improve the human immunity. Thus it has a high economic value and good development prospect. The climatic conditions of Taishan is suitable for the medicinal growth and there have many resources. In recent years, the resources are declining due to the ecological environment , natural conditions, and man-made destruction and plunde. Development and utilization the wild resources of versicolor in Taishan could not only protect the wild resources,but also improve the exploit value of medicinal fungi. So far, the biological characteristics of Taishan versicolor and domestication cultivation has yet been reported.From 2006 to 2009, the wild resources of versicolor in Taishan were detected and collected. Twenty-three strains were collected and three ones ( TN-1, TN-2, TN-3 ) that prossessing good traits were isolated and preserved by antagonistic test and fruiting tests. Our result showed that TN-1 strain was the best one, so we research it deeply. We identified the TN-1 strain is Coriolus versicolor (L.:Fr.) Quél. from the characteristics of morphological, mycelium culture and microscopic observation.Biological characteristics research showed that the optimal carbon source is maltose and sucrose, and the optimal nitrogen source is peptone and yeast extract. The primary and secondary order of mycelial growth factors is maltose, peptone, KH2PO4 and MgSO4. The mycelium of TN-1 strain can grow from 5℃to 35℃and the optimal growth tempreture was found to be 25℃-28℃. TN-1 grows faster under the dark condition . The optimal pH of mycelial growth was found to be 5.5 - 6.5.The optimal conditions in liquid culture of TN-1 were screened and the results are as followed.The temperature should be controlled at 25℃-30℃and the optimal temperture was found to be 28℃. The optimum pH value was 5-6. It could get the largest mycelium growth amount under the optimal tempreture and pH in 500mL bottle that filling 150mL liquid culture after being cultured six days .The optimal shake-flask speed is 120-150r/min. In this study, we obtain one versicolor strain by artificial domestication cultivation, The suitable medium for mycelial growth in different stages were screened. We also determined the the best fruiting condition such as fruiting method, the moisture of medium, capacity of loading feeding, fruiting temperature and humidity. In the conclusion, the artificial domestication cultivation technology system of versicolor was initially established . |
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