The Comparisons Between The PGC Expression And The PG Serum Level Among Different Gastric Disease Tissue, And Its Relationship With Hp

Objective Immunohistochemical method(SABC) was used to detect the expression levels of PGC in CSG,CAG,IM,DYS and GC tissue, variation in the same group before and after radical cure for Hp infection. To detect the level of sPGA, sPGC and the variation in serum pepsinogen concentration before and after Helicobacter pylori eradication by ELISA. To analyze the relationship between the PGC and Hp, the correlation between the expression of PGC and the level of sPGA, sPGC, which will help to guidance the clinical work , also further interpreted PGC may be a good molecular maker for the early diagnosis of gastric carcinoma .Method The experiment was performed from 2008 to 2009 at the Department of Pathology in Affiliated Hospital of Medical College of Yan'an University . The expression of PGC was detected by immunohistochemistry in 49 chronic superficial gastritis(CSG), 35 chronic atrophic gastritis (CAG), 40intestinal metaplasia(IM), 39 Dysplasia (DYS)and 41gastric cancer (Ca) in 204 endoscopic biopsy specimens. To detect the level of sPGA, sPGC by ELISA. The data were analyzed for significance using the statistical software SPSS 16.0 computer package. Chi-square test was used in the analysis of enumeration data, the differences of measurement data were compared with the analysis of variance and t test, Tissue expression and serum in correlation analysis using Person. P <0.05 statistically significant.Result1. PGC Expression among different gastric disease tissue there were differences in PGC Expression among different gastric disease tissue, the expression of pepsinogenC decreased significantly on sequence(P<0.05 ).the positive rate of PGC is 95.9%,77.1%,55.0%,51.3%,7.3% (CSG,CAG, IM,DYS,Ca). The positive rate of PGC in CSG was significantly higher than in the others(P<0.05), The positive rate was significantly higher in CAG than in IM,DYS and Ca, but there is no significant difference between IM and DYS(P>0.05). The positive rate was significantly lower in Ca than in others(P<0.05).2. Comparison Serum Pepsinogen Levels in patients with different gastric diseasesThere were differences in concentration of sPGA, sPGC among the groups. And there was extreme significant statistics difference (P< 0.05). sPGA decreased significantly on sequence(P<0.05). sPGC increased significantly on sequence(P<0.05).sPGC in Ca is higher than in others(P<0.05),but no significant difference in serum PGC were detected in CSG,CAG, IM,DYS(P>0.05).3. Comparisons between different expression of tissue PGC and concentration of serum PGBy the decrease of PGC expression, lesion tissue in CAG,CSG,IM,DYS,Ca, their concentration of serum PGC was increased and their sPGA concentration was decreased, and there is statistic difference(P<0.05). The sPGA decreased, the sPGC increased along with decrease of PGC expression in lesion tissue, they have good relativity and the statistic difference was similar (ra=0.956,Pa= 0.011;rc=-0.968,Pc=0.007).4. Comparisons between different expression of tissue PGC and concenrtation of serum PG in H.p infections and non-infectionsRate of PGC expression in groups (CSG,IM,DYS,Ca) of H.p infection was lower than that of non-infection(P<0.05),but there is no statistic difference in CAG(P>0.05). The rate of PGC increased significantly on sequence in different groups of eradication successfully (P<0.05),and in IM,DYS,there is statistic difference(P<0.05), but in CAG or CSG, there is no difference(P>0.05). The difference was not statistically significant in the gruoups of eradication failure (P>0.05).Concentration of sPGA, sPGC in groups of H.p infection was higher than that of non-infection (P<0.05). There is significantly difference of sPGC among the gruoups (P<0.05).but, only in CAG there is significantly difference of sPGA(P<0.05). Concentration of sPGA, sPGC is lower in different groups of eradication successfully than before (P<0.05), but the difference was not statistically significant in the gruoups of eradication failure (P>0.05).Conclusion1. the rate of PGC expression in different age and sex of gastric disease in patients with no significant differences. sPGA and sPGC is higher in male than in female, is higher in <65 guoups than in≥65 groups.2. Tissue expression of PGC is negative to the malignant degree of gastric mucosa cell and positive with the development of gastric of gastric mucosa diseases.3. Serum sPGA decreased obviously is an increased risk.4. The test, which only use sPGA or sPGC to detect gastric mucosa, isn't a marked in clinic, and at the same time the detection of sPG is relative to the tissue expression of PGC antigen, it is a convenient, economic maker to screen and diagnose gastric mucosa disease, and has some clinical value.5. the patients with H.p infection ,low sPGA or high sPGC and negative expression of PGC should be closely followed.6. Concentration of sPGA, sPGC is lower of eradication successfully than before (P<0.05).The rate of PGC increased significantly on sequence of eradication successfully in IM, DYS(P<0.05). It shows Hp perhaps can slow IM, DYS of these premalignant changes to the development of cancer.