Study On The Relationship Between The Serum Leptin And C-Reactive Protion With The Carotid Artery Atherosclerosis In Type 2 Diabetes Mellitus

Objective: This study aims to investigate the correlation between leptin, CRP and carotid atherosclerosis in type 2 Diabetes.Methods: According to the diagnostic criteria of atherosclerosis including plague, intima thickening, vascular sclerosis, vascular stricture and etc, we divided 61 patients that were clinically diagnosed with type 2 Diabetes into two groups. (1) T2DM with CAAS group (group A): 30 cases (17 males, 13 females), age:36-75 years (54.27±11.95), BMI(25.47±2.83)kg/m2. (2) T2DM group (group B): 31 cases (18 males, 13 females), age: 39-70 years(54.65±12.77), BMI(24.64±2.40)kg/m2. 31 healthy people, age: 30-70 years (average 54.10±9.69), BMI(24.98±2.03)kg/m2 were included as controls (group C). Using Enzyme linked immuno sorbent assay (ELISA ) and lympus immune agglutination nephelometry, we assessed leptin, CRP and double carotid vessels for the three aforementioned groups individually.Results:①The leptin levels were much higher in group A and B than group C, and had statistic significance in differences.(P<0.01), but had no significant differences between group A and group B(P>0.05). All three groups demonstrate:the leptin levels in females were higher than in males in each group(P<0.01).②CRP assessment: the density of plasma CRP in group A was (9.26±1.23)mg/L; group B: (7.01±0.69)mg/L;group C: (3.9±1.6)mg/L. the leptin level in group A and B were much higher than group C, the difference was significant(P<0.01), and that in group A was much higher than group B with statistic significance(P<0.01). The thickness of carotid intima media tunica (IMT) in group A was much higher than group B and C, and had significant difference(P<0.01); the IMT in group B was much higher than control group, and had significant difference(P<0.05).③The linear correlation analysis of plasma CRP, leptin and IMT in T2DM group revealed that plasma leptin(r=0.2642,P<0.05) and IMT(r=0.69,P<0.01) were positive correlated.Conclusions:①Plasma leptin, CRP are risk factors for insulin resistance and type 2 diabetes, and are correlated with insulin resistance and type 2 diabetes.②The plasma CRP level in T2DM patients had close relationship to carotid atherosclerosis, and continuous rises with the occurrence, development and formation of atherosclerosis plaque. The rise of plasma CRP level may be the marker for macroangiopathy in T2DM patients.③The IMT in DM patients with macroangiopathy were much thicker than control group(P<0.01), and had a higher plaque detection rate. Linear correlation analysis revealed that plasma CRP and IMT(r=0.69, P<0.01) were positive correlated.