| Objective: To investigate serum leptin and its relationship with the predisposing factors of vasculopathy in type 2 diabetes with coronary heart diseases.Methods: A total of 30 diagnosed type 2 diabetes mellitus patients and 30 diagnosed type 2 diabetes with coronary heart diseases from out-patient of QiLu Hospital in 2005-2006 and 30 non-diabetic volunteers from medical examination center were recruited in this study. 30 type 2 diabetes mellitus patients are diagnosed according to 1999 World Health Organization criteria on diabetes mellitus. Another 30 type 2 diabetes with coronary heart diseases patients are diagnosed according to 1999 World Health Organization criteria on diabetes mellitus and 1979 World Health Organization criteria on coronary heart diseases. Fasting plasma glucose, lipids, fasting insulin, C reactive protein, GHbAlC and leptin were measured. Body mass index and waist hip rate were calculated. Insulin resistance index was estimated by the insulin resistance index of homeostasis model assessment(HOMA-IR). The intima-media thickness of the common carotid artery was examined by a high resolution color doppler ultrasonography. The correlation of serum leptin and sex, age, BMI, WHR, FPG, lipids, FINS , HOMA-IR, CRP, IMT were analyzed. Descriptive statistics are presented as mean ± standard deviation(SD) for variables with a normal distribution. Serum leptin and insulin which submit abnormal distribution were analyzed after logarithmic transformation. Student's t-test was used to compare between data of two groups, analysis of variance(ANOVA) was used to compare data of all groups, and Student-Newman-Keuls(SNK) was used to compare between means. Univariate and stepwise mulitiple linear regression analysis were performed to identify independent factors affecting leptin and to estimate the final predictors of their variability. A value of P<0.05 was considered to be statistically significant. Alldata were analyzed by SPSS 13.0 statistical process. Results: 1. WHR> SBP, HDL-C\ GHbAlC, FBG> HOMA-IR^ CRP and IMT werehigher in type 2 diabetes mellitus than in normal subjects. LnFINSN GHbAlC\ HOMA-IR^ CRP^ IMT were higher in type 2 diabetes with coronary heart diseases patients than in type 2 diabetes mellitus and normal subjects. Serum leptin levels were significantly higher in type 2 diabetes mellitus patients than in normal subjects. Serum adiponectin levels were significantly higher in type 2 diabetes with coronary heart diseases patients than in type 2 diabetes mellitus patients.2. Serum leptin level was significantly higher in women than men in all groups.3. Correlation analysis suggested serum leptin levels were positively correlated with WHR, FINS,and were not correlated with age,SBP,DBP,HDL-C,FBG, GHbA1C .A stepwise mulitiple linear regression analysis showed that FINS,sex,CRPand IMT entered the regression equation. Conclusions:1. Serum leptin levels were significantly higher in type 2 diabetes withcoronary heart diseases patients than in type 2 diabetes mellitus patients and normal subjects , and were positively correlated with HOMA-IR.2. Serum leptin levels were significantly higher in women than men in all groups, which suggests serum leptin has sex differences.3. Correlation analysis showed that there was independent relationship with leptin to WHR and FINS. Stepwise regression analysis showed gender, FINS, CRP and IMT entered the regression equation in the final phase. |
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