An Experimental Study Of The ACTN3,ADRA2A,CNTF Gene Polymorphism Of Han Nationality Servicemen In PLA

Introduction: The person of different human race and different individual is difference in the exercise ability which already no one query. In recent years, it is confirmed that mankind's exercise ability difference contains the heredity foundation by the research result of mankind's genetics.The genetics research expresses that the outstanding sport ability has a higher heredity degree. The research confirms that the biggest oxygen ability of mankind have the heredity degree of 25%-90%.Along with modern molecular biology technical development, the research of choosing athlete member that have already went deep into genetics realm. These researches will develop a new path for the work of choosing athlete. Adding into the gene index sign in the choosing excellent athlete, which will gradually make it science and accurate in choosing work. Mankind's gene polymorphism researches bring revolution influence in the physique research. Passing this "strategic" research of polymorphism of gene, we can be make use of in the military medical science research, raise the quality in choosing the special kind soldier's work, draw up a project to have real meaning of characteristic training, prevent the occurrence of the training wound better. There have some genes having something to do with the exercise character , the gene CNTF,ACE,mtDNA,PGC-1αetc have related to endurance, the gene GDF8,VDR has something to do with strength, the gene ACTN has relation with speed. This experiment chooses among them of three kinds of genes, the application of fluorescence PCR technique carries on initial analysis. These three kinds of gene have related to speed, endurance and strength of individual respectively , try to explore the heredity about the exercise character in many angles.Objective:To explore the characteristics of polymorphisms in ACTN gene phenotypes and their relationship to the speed capacity in PLA servicemen of the Han nationality. To explore the characteristics of polymorphisms in ADRA2A gene phenotypes and their relationship to the aerobic training in PLA servicemen of the Han nationality . To explore the characteristics of polymorphisms in the CNTF gene phenotypes and their relationship to the muscular capacity in Han nationality servicemen in PLA .Methods:One hundred and thirteen healthy PLA recruit of the Han nationality in China underwent a 100-meter sprint convention training program for 18 weeks, and then got a test of 100-meter running. The ACTN3 allelic variant and genotype was analyzed by Q-PCR. The relation between the result of 100-meter sprint and the polymorphisms of ACTN3 .One hundred and eight healthy PLA recruit of the Han nationality in China underwent a 5000-meter racing convention training program for 18 weeks, and then got a test of 5000-meter racing. The ADRA2A allelic variant and genotype was analyzed by polymerase chain reaction and sequencing, the characteristics of polymorphisms in three sites of G6412C,T6623A and C6645C were determined with biochemical technique. The relation between the result of 5000-meter racing and the polymorphisms of ADRA2A .One hundred and five healthy PLA recruit of the Han nationality in China were recruited to undergo convention training program for 18 weeks, and to examine grasp-force/weight index. The CNTF allelic variant and genotype was analyzed by Q-PCR. The relation between the result of grasp-force/weight and the polymorphisms of CNTF .Results:1.Genotype of ACTN3 C1747G distributions was consistent with Hardy-Weinberg equilibrium at this loci .The genotype frequencies of C/T,C/C,T/T were 43%,40%,17%,respectively.The allele frequencies of C and T were 62% and 38%. There was not significant difference in frequency distribution of ACTN3 polymorphism between Han nationality and European white men (P>0.05). The speed of 100-meter running of recruits carrying T/T (12.98±0.65s) was significantly faster than that of those carrying C/T (13.45±0.78s, P<0.05) and C/C (13.50±0.86s, P<0.05) .2.Genotype distributions at all the three locations were consistent with Hardy-Weinberg equilibrium. The genotype frequencies in T6623A of A/G(50%) was higher than that of G/G(26%) and A/A(24%).The allele frequencies of A(51%) was higher than that of G (49%). The 5000-meter racing time of recruits carrying G/G (20.95±0.82min) was significantly longer than that of those carrying A/A (18.97±0.65min, P<0.05) . The 5000-meter racing time of recruits carrying G/G (20.21±0.73min) had no significant difference compare with that of those carrying A/A and A/G. There was not significant association between the aerobic capacity and the polymorphism of G6412 and C6645C.3.Genotype of CNTF distributions was consistent with Hardy-Weinberg equilibrium at this loci . There was significant difference in sex in China(P<0.05), There was not significant difference in frequency distribution of CNTF polymorphism between Han nationality and European white men (P>0.05).And men carrying G/G were at higher level than that carrying A/A and A/G. A significant difference in muscular capacity across genotypes was not observed at CNTF (P=0.16).Conclusions:①The C1747G site of ACTN3 was a gene maker for predicting speed capacity.②The T6623A of ADRA2A was the ideal gene maker for predicting endurance capacity ,but G6412C and C6645C was not.③This research concluded that in consideration of the factors of racial difference,limited samples and age,this research can be the representative of frequency distribution of CNTF polymorphisms of group of Chinese Han people.It was suggested that the CNTF was not gene markers for predicting muscular capacity.