The Improving Solid BANA Test And Its Application In Detecting The Periodontitis

Objectives: P. gingivalis (Pg), B. forsythus (Bf) and T. denticola (Td), the three well known putative periodontopathogens can produce trypsin-like enzyme which is one of the important destructive factors of periodontal tissue. The hydrolyzing activity of trypsin-like enzyme on bengoyl-DL-arginine-naphthlamide (BANA) can be measured with routine liquid BANA test, a relative complicated techinque. The aim of this study at the first is to detect the sensitivity of solid BANA test and find the optimal working conditions, establish a practical and stable solid BANA test technique; and at the second is to assess the value of the solid BANA test in clinical periodontal use and to reveal the possible correlation of the results of the test with parameters widely used in Periodontics at sites in patients with an initial diagnosis of adult periodontitis.Materials and Methods:1 Bacteria: The tested strains include 14 strains of oral anaerobic species and 6 strains aerobic bacterias. Bacterias were cultivated at respectively condition.2 Samples collection: Patients were selected at the stomatology department of Bethune International Peace hospital.