The Study Of The Detection Rate Of 7 Kinds Of Microorganism From Subgingival Plaque In Patients With Different Types Of Subgingival Plaque Of Periodontal Diseases

PURPOSE.The purpose of the present study was to detect the existence of Aa,Pg,Tf,Cr,Ec,Pn and Archaea,which are related to periodontal status.METHODS:Dental plaque from 120 subjects from periodontal division in Yantai stomatological hospital was sampled, including 40 volunteers with health periodontal status(Group A),40 patients with dental plaque-induced gingival diseases(Group B) and 40 patients with moderate or severe chronic periodontitis(Group C). These samples were detected based on bacterial composition using the terminal restriction fragment length polymorphism of 16S rRNA genes by multiple-Polymerase Chain Reaction,then used chi-squaretes test for the stasistical lanalysis.RESULTS:The detection rate of Pn,Cr and Pg have statistical differences between group A and B. The detection rate of Ec,Cr,Pg,Aa,Tf and Archaea have statistical differences between group C and B. The detection rate of Ec,Pn,Cr,Pg,Aa,Tf and Archaea have statistical differences between group A and C.CONCLUSIONS:The rate of Ec,Pn,Cr,Pg,Aa,Tf and Archaea detected in patients with moderately severe dental plaque-induced gingival disease was much higher than that of healthy subjects, illustrating that these bacteria have certain correlation with severe chronic periodontitis.The rate of Ec,Cr,Pg,Aa, Tf and Archaea detected in severe chronic periodontitis was significantly higher than that in dental plaque-induced gingival disease, showing their close relationship with periodontal disease progression. The rate of Pn,Cr,Pg detected in dental plaque-induced gingival disease,showing their close relationship with the sensitivity of the gingival inflammation.