The Distribution Of Periodontal Pathogens In The Subgingival Plaque And The Coronary Atherosclerotic Plaque

Objective:To investigate the possible correlation between coronary heart disease and chronic periodontitis.Methods:Sub-gingival plaque samples and coronary atherosclemtic plaques were collected from 36 patients(13 Uygur cases,23 Han cases) who scheduled for coronary artery bypass surgery with periodontitis.50 sub-gingival plaque samples were collected from patients with chronic periodontitis. The DNA of bacteria was obtained from sub-gingival plaque samples and coronary atherosclerotic plaques by using chelex-100 method, and then five kinds of bacteria which correlated to periodontitis, such as Pg(Porphyromonas gingivalis),Aa(Actinobacillus actinomyce-temcomitans),Fn(Fusobacterium nucleatum),Pi(Preuotella intermedia),Tf(Tannerella forsythensis), were detected by polymerase chain reaction technique(PCR). Results:1) The positive cases of five kinds of bacteria detected respectively in subgingival plaque of CHD group(n=36)was:Pg39%, Aa 19%, Pi 75%, Fn 25%, Tf 92%. The positive cases of five kinds of bacteria detected respectively in control group(n=50)was:Pg30%, Aa 8%, Pi 14%, Fn 25%, Tf 85%. The detective ratio of Pi in coronary heart disease group was significantly higher than control group. There were statistically significant difference between two groups above(P<0.05); 2) The CHD group(n=36)and the control group(n=50)both had the highest detective ratio of Tf and Fn; 3) The detective ratio of five bacteria respective in coronary atherosclerotic plaques samples from 36 patients was, Pg36%, Aa 3%, Pi 14%, Fn 25%, Tf 47%; 4) The cases of the same periodontal bacteria which were detected both in subgingival plaques and in coronary atherosclerotic plaques of the same patient were:Tf 15, Pg 13, Fn5, Pi 3, Aa 0. Conclusions:1) There is possible correlation between Pi(Prevotella intermedia)and coronary heart disease. Pi(Prevotella intermedia)may be the risk factors for coronary heart disease; 2) Tf and Fn may play an important role in the development of coronary heart disease; 3) Chronic periodontitis may correlate to coronary heart disease. The periodontal pathogenic bacteria may play an important role in the initial stage and the process of coronary heart disease.