The Expression And Significance Of Apoptotic Modulating Genes Fas,Bcl-2 MRNA Of PBMC In Patients With MG

Objective: To expore the relationship of Fas, Bcl-2 mRNA in PBMC of patients with MG. Methods: The levels of Fas, Bcl-2 mRNA were detected with semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) technology in 45 patients with MG and 30 healthy donors served as a normal control (NC).MG group was subdivided into subgroups respectively according to their absolute scores rated by Xu's score system and Osserman typing system. Results: (1)The level of Fas mRNA in MG group was not lower compared with that of NC group.The level of Bcl-2 mRNA in patients was higher than those in NC group (P<0. 05), especially in the cases of â…¢,â…£ type(P<0. 05, P <0. 01). (2) The levels of Bcl-2 mRNA in moderate, serious, and much serious groups were higher than that of slight group(P<0. 05, P<0. 01).And there was a positive correlation between the level of Bcl-2 mRNA and myasthenic scores (P<0. 01) .(3) After therapy of glucocorticoid, the level of Fas mRNA was not changed compared to before treatment, while the absolute scores and the level of Bcl-2 mRNA were significantly decreased (P<0.01). Conclusions: MG PBMC Bcl-2 might be involved in the pathogenesis and progression of MG while the level of Fas mRNA might be not. The level of Bcl-2 mRNA was a reliable parameter in evaluating the disabilities in MG patients. And it was also usefull in monitoring the effect of therapy on MG .