The Experimental Study On Treatment Of Keloids For Animal Models By β-ray

Objective:To establish animal models for keloids in rabbits and to observe the changes of keloids ears of rabbits by exposure of β -ray(emission from 90Sr). To get the most effective dosage and time in treatment of keloida with β -ray.Methods: To total 40 ears of 20 rabbits.and 320 square wounds (6 x 6mm) with total skin loses on the ventral side were created. Eight wounds were created in every ear. The 20 right ears were control group. The wounds healing time and the excessive dermal scars were observed. The 20 left ears left ears were experimental (exposure) group, divided randomly into 5 groups . The 5 experimental groups were exposed with (3 -ray on 4 8 12 20 28 days after wounded,exposuer doses were 600 1000 1500 3000cGy( one time ).A11 wounds were reoperated and observed for microscope and electron microscope. Result: Excessive dermal scarring appeared in 131 wounds(81.9%131/160),and reach the top in about a month after wound .These were leloid models.There was no significant difference in heeling times between the experimental groups and control groups., however there was significance among difference β -ray dose which affect the mount of in keloids (F=144.71 p < 0.001 ) .The fibroblasts cell was no difference between 12 and 28 days after wounded (p >0.05). Conclusions: Excessive dermal scarring appears after wound on the ventral side of rabbit's ear which were similar to the keloid of human being in morphology and pathology and cell ultrastructure without stimulation. It can become an animal model for the study of keloid. Exposure of β -ray could treat keloid: It was 3000cGy β -ray that can effectively prevent the growth of keloids within a week after wounds healing.