| Objective:Vascular Dementia (called VD in short ) belongs to the disease scope of dementia of old age in the light of tradditional Chinese medincine(TCM).In the recent years,with the aging of population the incidence of vascular dementia is on the rise and affects seriously the people's working ability as well as living quality.Therefore,it's of great significance to make deeper research on it's pathological mechanism and develop the effective medicine with the integration of both Chinese and western medical theory.In order to find the relationship between disequilibrium of oxidation--anti-oxidation and Vascular Dementia ,then further to analyse the therapeutical pathogenicity with DangguiShaoyaoSan,we detected malonaldehyde(MDA),superoxide dismutase(SOD),glutathione(GSH) and glutathione peroxidase(GSH-Px) of cerebral tissue on mice with Vascular Dementia . Methods:　360 Kunming mice were divided into six groups at random,which were sham-operation group(which were abbreviated to "S group"in the following),model group(which were abbreviated to "M group" in the following,high dose of DangguiShaoyaoSan group(which were abbreviated to "H group" in the following), low dose of DangguiShaoyaoSan group(which were abbreviated to " L group" in the following), extract of Ginkgobiloba control group(which were abbreviated to "E group")and Nimodipine control group(which were abbreviated to "N group" in the following). There were 60 mice in each group. We duplicated the model of vascular dementia mice with the operation of cerebral ischemic reperfusion except S group.　And then 6 group were continuously filled medicine or saline through the stomach from the second day after the operation of cerebral ischemic reperfusion.　S group and M group were filled saline and H,　L,E and N group were given medicine. On the 7th,15th and 30th day after praxiology experiment,the mice were cut head for obtaining cerebral tissue, the left part of which was fixed by 10% for maldehyde solution. The tissue was stained by HE and Thionine. 　　We observed constitutional morphologic changes of cerebral tissue under light microscope and the right part of which were made into tissue homogenation for check up. The experiment used TBA compared color analytical method to detect the content changes of MDA in cerebral tissue of Kunming mice, and used xanthine oxidase method to detect the activity changes of SOD, and used compared color analytical method to detect the activity changes of GSH and GSH-Px in cerebal tissue of Kunming mice. WithGinkgobiloba extract and Nimodipine as the control medicine,we observed the effect of high and low dose of DangguiShaoyaoSan on MDA and GSH content and the activity of SOD and GSH-Px in cerebral ischemic reperfusion. Then we detected the mechanism on vascular dementia with DangguiShaoyaoSan from the direction of anti-oxidation injury.Results:1 Results of praxiology experiment1.1 The mice's performance record of water-labyrinth There was no significant difference between the learning and memorizing performance. The wrong times and the time of swimming along the whole line of M group was more than that of S group (P<0.05or P<0.01).　At the same time,　the swimming time and the wrong times of H ,L,E and N group was less than that of M group(P<0.05or P<0.01). Comparing with those of L,E and N group ,The swimming time and the wrong times of H group was less on the 15th and 30th day(P<0.05or P<0.01),but not on the 7th day after the operation of cerebral ischemic reperfusion(P>0.05).There was no statistical difference among L, E and N group according to the learning and memorizing performance record ( P>0.05).1.2 The mice's performance record of water-labyrinth Experiment of jumping stand M group has shorter reaction time(the time of jumping up the stand), longer latency time(the time of jumping down thestand) and more wrong times than S group (P<0.05or P<0.01).At the same time, H group has longer reaction t...
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