| Objective: Chronic bronchitis is a kind of common and frequently-occurring disease in respiratory system. There were typical oxidative injury phenomena during its process. Along with the clinical experience of my tutor, our project group on "experimental and clinical investigation on chronic bronchitis with Kechuanning capsule " selected effective medicine to manufacture Kechuanning capsule according to the main disease cause and pathology. In order to find the relation between disequilibrium of oxidation-anti-oxidation and chronic bronchitis, then further to analyse the therapeutical pathogenicity with Kechuanning capsule , we detected malonaldehyde (MDA) superoxide dismutase (SOD) catalase (CAT) glutathione (GSH) and glutathione peroxidase (GSH-Px) of serum lung tissue and bronchoalveolar lavage fluid (BALF) on rats with chronic bronchitis.Methods : 40 SD rats were put in experimental circumstance with temperature of 11 17 for a week before experiment and were divided into five groups at random, which were normal control group ( which were abbreviated to " normol group "in the following), model controlgroup (which were abbreviated to " model group " in the following). low dose of Kechuanning capsule group (which were abbreviated to " low dose group " in the following ). high dose of Kechuanning capsule group ( which were abbreviated to "high dose group" in the following) and Guilong capsule control group ( which were abbreviated to "Guilong group" in the following, another medicine of the similar kind). There were four female rats and four male rats in each group. We duplicated the model of chronic bronchitic rats with refined smoking-fumigated method in accordance with experimental method of medical animal. 32 rats in manufacture-model group were put in a special smoke-made room with an area of Inr in which paring sawdust and tobacco leaf 50g respectively were lit to smoke for 30 days, with 2 times each day and 30 minutes each time. Normal group were bred in common circumstance but smoke. From the second day after model were produced succssfully , 5 groups were continuously poured medicine or saline into stomach for 21 days. Nomal group were poured saline l0ml/kg, 1 time a day; model group were poured saline lOml/kg, 1 time a day; low dose group were given medicine 0.9g/kg ( 14.1 times as much as clinical adult dosage) and poured in suspension fluid form with 0.09g/mh 1 time a day; high dose group were given medicine 1.8g/kg ( 28.2 times as much as clinical adult dosage) and poured in suspension fluid form with 0.18g/ml, 1 time a day; Guilong group were given medicine 0.9g/kg (14.1times as much as clinical adult dosage ) and poured in suspension fluid form with 0.09g/ml, 1 time a day. The second day after treatment all rats were cut head for abtaining blood, which were centrifugated for 5 minutes ( 3500r/min ) immediately. We saved serum to freeze at-20 for check up and took an area of 0.5cm 0.5cm from the middle tissue of left lung, which was fixed by 10% formaldehyde solution. The tissue section was stained by HE. We observed constitutional morphologic changes of lung and bronchi under light microscope and flushed the remnant tissue of left lung by freezed saline and sucked water on it by filter paper, then weigh out a piece of 0.3g wet tissue and put it in prepared cold saline. We used the electric homogenate utensil to dispense the wet tissue into 10% lung tissue homogenatio centrifugated for 5minutes (600.0r/min) at-TC, and extracted the above fluid to save freezed at-20 for check up. We took the right lung tissue to bronchoalve lavage (BAD and washed the surface blood with saline at -TC for 4 times repeatedly, 4ml saline each time. The recovery volume was above 90% , which was centrifugated for 5 minutes ( 1500r/min) at4, then extracted the surface fluid to save freezed homogenate at-20 for check up.The experiment used TBA compared color analytical method to detect the content changes of MDA in serum lung tissue and BALF of SD rats: and used xanthine oxidase method to detect the activity...
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