Study On The Multiplication And Rooting Transplantation Of Paeonia ×lemoinei 'High Noon' And P.ostii 'Fens Dan'

Although some advances have been made about the tissue culture in vitro of tree peony,the protocol is limited in commercial use due to the poor capacity for shoot multiplication,low frequency of in vitro rooting,and the great losses during ex vitro acclimatization.In this study,the tissue culture in vitro of the Paeonia×lemoinei 'High Noon',P.ostii 'Feng Dan'(FD1?FD6)were further research,in order to improve the protocol for the tissue culture system of tree peony.Main results were as follow:1.Initiation:The modification of WPM medium added to MT(Meta-Toplin)0.5mg·L-1and GA30.2mg·L-1 were suitable for initiation of Paeonia×lemoinei 'High Noon',P.ostii Feng Dan',and the germination rate was 100%.The explants type of Paeonia×lemoinei 'High Noon' were significant influence on the pollution and multiplication.(The buds pollution and multiplication rate were more than stem segments)2.Multiplication:Response surface analysis was applied to optimization the culture medium for the Paeonia×lemoinei 'High Noon'.The optimal medium components were macro-element 75ml/L,micro-element2.5ml/L,Ca75ml/L,organic matter 2.5ml/L,Fe-EDTA 4ml/L,inositol 7.5ml/L and the multiplication rate improved 10%.The NAA and dark culture weren't suitable for multiplication of Paeonia×lemoinei 'High Noon',while as an additive organic,the Lactalbumin Hydrolysate of 100mg.L1 was good for the shoots growth.The multiplication rate was gradually decrease with the increasing of subculture times.Using the BA over two times,shoots was multiplication down and growth slowly.But MT and BA alternate can avoid this phenomenon.The WPM(Ca-+3×)+BA0.3mg.L-1+GA30.05mg.L-1+KT0.1mg.L-1 medium was best to the multiplication of FD],multiplication rate was 2.56,and the subculture time was 30?35d.3.In rooting:Using the 0.3mg.L-1 PP333 rejuvenation culture 10d,before root induction duration,was good for improve the rooting rate(51.22%)and quality of Paeonia xlemoinei 'High Noon'.However,anti-browning agents,rutin,potassium fulvate were not suitable for rooting.The orthogonal experiment of WPM medium in the root induction can improve the rooting rate in the subculture cycle of 19?20,while the subculture cycle of 5 was useless.The 1/2WPM+NAA0.3mg.L-1+IBA 1.0mg.L-1+Putl.Omg.L'1 was best root induction medium of Paeonia×lemoinei 'High Noon'.1/2WPM+NAA0.1mg.L-1+IBA0.5mg.L-1+Put1.Omg.L-1 was best root induction medium of FD6 and the rooting rate was 43%.1/2WPM+IBA1.0mg.L-1+Putl.0mg.L-1 was best root induction medium of FD1 and the rooting rate was 64%.4.Acclimatization:Different treatments affected significantly influence the survival and growth of plantlets.And the cold treatment was the key to survival.Resection root tip can improve lateral root in the root formation.Treatments of Glomus mosseae in the transplant have different effect between varieties.Which was good for Paeonia×lemoinei 'High Noon' and the survival rate was 72%and better than FD6.