| Objective:1.To observe the clinical effect of Liangxue Qushi Decoction(LXQSD)in the treatment of acute eczema.2.To explore the possible mechanism of LXQSD by using mouse eczema model lesion,the difference of swelling degree and thickness difference,IL-2,IL-4 and LTB4 levels,AQP3 mRNA gene and protein expression.Methods:The subject study was conducted in two parts:1.Decoction of Liangxue prescription clinical observation.To investigate the diagnostic criteria of acute eczema to design randomized,open and parallel controlled trials.To control the erythema,pimples,blisters,exudation,target lesion area and itching To assess the overall clinical efficacy,and to observe the recurrence rate and adverse drug reactions.2.Explore the mechanism of LXQSD:(1)To observe the morphological changes of skin lesions in mice with eczema.The model of eczema was induced by 2,4-dinitrochlorobenzene,for LXQSD drug intervention,were treated with LXQSD control group 3 negative control group(physiologic saline)and blank Group.According to Zhao’s EASI score method to score to evaluate the efficacy of erythema,edema,exudation,epidermal exfoliation and other skin lesions changes.(2)The mice were treated with drug after treatment,determine edema degree and thickness difference about the groups.(3)The levels of IL-2,IL-4 and LTB4 in the skin lesions of the eczema model were determined by ELISA.The levels of IL-2,IL-4 and LTB4 in the eczema model were determined by ELISA.(4)RT-PCR was used to detect the content of AQP3 mRNA in the eczema model of LXQSD group,loratadine group,model group and blank group.(5)Western Blot method was used to detect the expression of AQP3 protein in the patients with LXQSD group,loratadine group,model group and blank group.result:1.Clinical curative effectThe treatment of acute eczema was significantly better than that of loratadine group(P<0.05),and the difference was statistically significant(P<0.05).There was a significant difference(P<0.05)in the area of lesion area and loratadine group(P<0.05).In the degree of pruritus,LXQSD group significantly better than loratadine group(P<0.05).For the two groups of the overall efficacy evaluation,the total effective rate of LXQSD group was 86.49%,the total effective rate of loratadine group was 80.56%,by statistical analysis,(P<0.05),LXQSD the overall efficacy of the group was better than that of loratadine.2.Basic experimental study(1)Morphological observation:LXQSD was used to control the eczema model of mice.Compared with loratadine and model group,there were significant differences in erythema,papules,exudation and epidermis exfoliation(P<0.01),and the difference between the two groups was significantly higher than that of the model group(P<0.01),and the patients were divided into two groups The efficacy of the index was significantly better than that of loratadine(P<0.05).In the overall curative effect evaluation,the total effective rate was 88.89%,78.78%in the loratadine group and 27.78%in the model group,and the overall curative effect of LXQSD group was better than that of the groups.(2)Edema degree and thickness difference:In this study,there were significant differences(P<0.01)between the four groups in edema degree and thickness difference.Compared with the blank group,the thickness of the three groups had significant difference(P<0.01),compared with the model group,there was significant difference between the two groups(P<0.05)(P<0.05).The difference was statistically significant(P<0.05).(3)Levels of IL-2,IL-4 and LTB4:There were significant differences in IL-2,IL-4 and LTB4 levels in the patients with LXQSD,loratadine,model and blank P<0.01).Which was lower than that of the blank group(P<0.05).The level of IL-2 in the LXQSD,loratadine group was higher than that in the model group(P<0.05)LXQSD group was significantly increased(P<0.05).higher than that in the blank group(P<0.01),the level of IL-4 and LTB4 was significantly lower than that of the control group(P<0.01),the difference was statistically significant.(4)AQP3mRNA genes:There was significant difference between the two groups(P<0.01).There were significant differences between the two groups(P<0.01).There were significant differences in the AQP3 mRNA level(P<0.01).Compared with loratadine group,the patients were treated with LXQSD group and loratadine group(P<0.01),the difference was statistically significant.(5)AQP3 mRNA expression level:four groups of bands clearly visible,gray-scale difference can be seen in the strongest model group,followed by loratadine group,once again LXQSD group,the lowest gray for the blank group The The AQP3/β-actin values were statistically analyzed.Compared with the blank group,there were significant differences between the three groups(P<0.01).The LXQSD group and the loratadine group were significantly lower than those in the model group(P<0.01).The difference was statistically significant(P<0.05),and the difference was statistically significant(P<0.05).Conclusion:The clinical effect of LXQSD is siginificant,which can eliminate the effect of erythema,blisters,pimples and reduce oozing.The basic experiment can reduce the degree of skin lesion swelling,reduce erythema,Epidermal exfoliation and other inflammatory response,the effective regulation of skin lesions in the IL-2 rise,down-regulation of IL-4 content,inhibition of skin lesions LTB4 release,effectively inhibit the model lesions AQP3 expression.Consider LXQSD has obvious anti-inflammatory effect and the role of recovery barrier function.It can be seen,LXQSD can adjust the balance of Th1/Th2,regulate the immune response,inhibit the inflammatory response.Can initially show that the role of LXQSD may be used to regulate immune function and anti-inflammatory mechanism.This study found that AQP3 can significantly inhibit the role of acute eczema,so as to achieve the function of repair barrier to reduce the role of seepage.It show that the role of LXQSD may be to restore the skin barrier function. |
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