Clinical And Laboratory Study Of Skin Barrier Function In Patients With Neurodermatitis And Subacute Eczema

Objective:Testified afunctional degree of skin barrier by measuring skin sebum level and skin hydration degree in lesional skin of patients with Neurodermatitis and Subacute eczema and analised the expression of Keratinl7and water channel protein Aquaporin-3in lesional skin to confirmed the afuction. consequently, took corresponding therapy by oil and water adjuvant suppliment to observe the effects on skin barrier repairment.Methods:Evaluated skin sebum level and skin hydration degree in36cases of patient with neurodermatitis,40cases of patient with subacute eczema and30cases of control subjects by noninvasive skin physiology function tester SOFT5.5. Treated subacute eczema group with compound allantoin cream and nitrofurazone ointment and neurodermatitis group with compound allantoin cream and Calcipotriol ointment. Analysed positive expression of K17and AQP3in18cases neurodermatitis,20with subacute eczema, and12healthy controls by Envision immunohistochemical technique, analyze experimental results by statistical software SPSS16.0.Results:1. Skin sebum level and skin hydration degree of patients were different with control group before treatment and the difference was significant (P<0.05)2. Skin sebum level and skin hydration degree of subacute eczema group were higher than that of neurodermatitis group before treatment and the difference was significant (P<0.05)3.Skin sebum level and skin hydration degree of clinical cured group with neurodermatitis and subacute eczema were higher than that of effective group, improved group and inactive group after treatment and the difference was significant (P<0.05).4. Skin sebum level and skin hydration degree of clinical cured group with subacute eczema compared with that of clinical cured group with neurodermatitis had no significant difference (P>0.05).5. Skin sebum level and skin hydration degree of clinical cured group with neurodermatitis and subacute eczema were respectively compared with that before treatment, the difference was significant (P<0.05).6. The expression of K17and AQP3in patients were compared with that of control group and the difference was significant (P<0.05).7. The expression of K17in subacute eczema was higher than that in neurodermatitis and the difference was significant (P<0.05).8. The expression of AQP3in neurodermatitis was higher than that in subacute eczema and the difference was significant (P<0.05).Conclusions:1. There were differences on the skin sebum level, hydration degree and expression of K17and AQP3between the patients and control group, imply the skin barrier in patients afunction.2. Subacute eczema groups skin sebum level and hydration degree were higher than that of neurodermatitis group, implys that the skin barrier afunction in neurodermatitis was more serious than that in subacute eczema.3. Applied moisturizing and emollient adjunctive therapy in neurodermatitis and subacute eczema skin, and the effects of verifing the sebum level and hydration were obvious.4. After clinical cured, the afunction of skin barrier in neurodermatitis and subacute eczema reconstructed and function.5. The dried symbol of neurodermatitis was associated with significant upregulation of water channel protein AQP3expression. The erythema and inflammation of subacute eczema also coincides with significant increasing of keratin K17expression.