The Exploration On The Pharmacodynamics And Mechanism Of Portulaca Extracts On Treating Acute Eczema

Objective To investigate the impact of the Purslane on the eczema skin, explore the effect and mechanism of single Purslane on the repairing lesion site of eczemaMethods1.70SD rats were randomly divided into control group, model group, Caspase-14gene knockout group, positive group and high, medium, low dose of Purslane group. Except The normal group, the hair of rat’s abdomen were shaved by electric clippers and electric shaver, the area is2cm X3cm, then,2,4-dinitrochlorobenzene was used to make rats acur first sensitization. After5days,2,4-dinitrochlorobenzene was used again on the right back of rat, the area is2cm X2cm, the left back as the control site. To use excitation on rat every3days,3times in a row. At the9th day, the right back has erythema, edema, erosion or exudative papules, it showed eczema model was made successful.21days later, all animals were sacrificed, eczema skins were taken out, analytical balance was used to measure swelling of eczema skin, QRT-PCR was used to detect the expression of Caspase-14and Filaggrin gene in each experimental group rat skin. Immunohistochemical detection was ued to measure TNF-α、IL-22、IL-4. IFN-γ2.50SD rats were randomly divided into control group, positive group and high, medium, low dose of Purslane group. The recommended external dosage were given. At the experimental day,0standard Sandpaper was used to scrape the right leg of rat, then, all drug were applied again, after10minutes, the0.01%histamine phosphate was applied on the scraping area, more and more concentrated histamine phosphate were used every3minutes,0.01%,0.02%,0.03%,0.04%...The concentration is the itch threshold when the rat lick the right foot back. Results:1. Compared with the blank control group, swelling of high dose group was significantly less than the control group (P<0.01), The medium dose group, positive drug group is lower than model group (P<0.05).2. Compared with the normal group, Caspase-14and Filaggrin cells’ value in skin tissue of model group and knockout was significantly decreased (P<0.01). Compared with the medel group Caspase-14gene knockout group, Caspase-14and Filaggrin gene in the three dose of Purslane groups was significantly increased (P<0.01).3. Compared with the normal group, IOD of TNF-α, IL-22, IL-4of model group was significantly increased (P<0.01), the IOD of IFN-γ was significantly decreased,(P<0.01); but the IOD of TNF-α, IL-22, IL-4of high and medium group was decreased (P<0.05), IFN-γ was decreased,(P<0.05);4. The histamine phosphate in different dose groups of itching threshold records showed the medium group was higher than positive group(P<0.05), the high group was significantly higher than positive group(P<0.01),Conclusion Purslane extract can constrict the blood vessels, inhibit capillary permeability, inhibit the inflammatory swelling, reduce the itching and swelling symptoms of eczema edema. Purslane extract can raise the expression of Caspase-14and Filaggrin of skin barrier system, it means Purslane extract can improve gene expressionthe of Caspase-14to accelerate dephosphorylation of ProFilaggrin to transformed into filaggrin, accelerating the hydrolysis of Filaggrin into a variety of natural moisturizing factor to enhance skin protective function. At the same time, Purslane extract decrease the expression of TNF-a and IL-22in the eczema, and increase the secretion of IFN-γ to inhibit IL-4, then, the cellular immune function was enhance.