| Objective1Pulse electro-acupuncture treatment compared with musical electro-acupuncture in the clinic trials to investigate the effectiveness of depression with somatic symptoms of gastrointestinal tract, To investigate whether music electro-acupuncture is a advantageous method.2To observe the effect of pulse electro-acupuncture and musical electro-acupuncture of "Baihui"(GV20)and"Yintang"onbehaviorobservation,small intestinalpropulsion rate,brain-gut peptide levels and mortorphological changes of rat hippocampal neurons in depression model rats so as to provide experimental evidence for acupuncture treatment of gastrointestinal symptoms relatingto depression while comparingthe effect of pulse electro-acupuncture with musical electro-acupuncture.Methods1The66Patients were divided into2groups,one group accepted musical electro-acupuncture treatment,another pulse electro-acupuncture treatment.HAMI) and Chinese Medical Syndrome scales have been applied in the evaluation before and after the treatment.2Sixty SD rats were randomly divided into control.model.Prozac. Pulse electro-acupuncture and musical electro-acupuncture groups,with12rats in each group.Depression modal was established by chronic mild stimulation combined with isolated raising.For rats of the Prozac group were treated by gavaga of Prozac.once daily for21days.The rats of thePulse electro-acupuncture group."Baihui"(GV20) and "Yintang"(EX-HN3) were punctured and stimulated by pulse electro-acupuncture, once daily for21days. While the rats of theMusical electro-acupuncture group were punctured the same acupoints and stimulated by musical electro-acupuncture.Therat’sbodyweight.open-fieldtest,andrelativevolumeof sucrose-intake wererecorded one day before and during experiments.After the behavior observation,G AS,NPY,CGRP and β-EP in the rats’colonic mucosa tissues and hypothalamus were detected by Radiommunoassay or Elise detection method.Small intestinal propulsion rate were recorded. Whilethe ultra structure of the hippocampus and colonic mucosa tissues were observed by Flection Microscope.ResultsClinical research1camparing pulse group with musicalgroup, there was no significant difference of curative effect at the end of2th,4th,6th week,while no significant difference of overall resultis at the end of treatment(P>0.05). 2The HAMD score were significantly improved in both pulse group and music group. the improvement presented at4week in pulse group,while2week in music group (P<0.05) significant improvement presented at6week in pulse group,while4week in music group (P<0.01)3The HAMD score of music group was much lower than the pulse group at4,6week music group (P<0.05)4At the end of6week,the score of depression, difficulty falling asleep, poor sleep quality, wake early, Mental anxiety, somatic anxiety, gastrointestinal symptoms, systemic symptom and weight reduction in both musical and pulse group changed significantly camparing with prior treatment (P<0.05). Significant improvement of difficulty falling asleep, poor sleep quality, wake early and gastrointestinal symptoms presented in musical group camparing with pulese group (P<0.05)5The score of Chinese Medical Syndrome decreased Significantly at the end of4,6week (P<0.05),and musical group was better than pulse group (P<0.05)6the score of depression, decreased appetite, distending pain in hypochondrium, abdominal distension, abdominal pain and loose stools in both musical and pulse group changed significantly camparing with prior treatment (P<0.05). Significant improvement of decreased appetite, abdominal distension, abdominal pain and loose stools presented in musical group camparing with pulese group (P<0.05)7There were no serious adverse events during acupuncture,some slight adverse reaction was easy to eliminate by corresponding measures.experimental research1Before the experiment,there is no diffence among these groups. At the end of the experiment, there are different. Compared with control group rats,body weight and sucrose intake of model group obviousely decreased (P<0.01),and crossing numbers, rearing times of model group decreased (P<0.05).2Compared with model group, crossing numbers and rearing times of pulse group,musical group and Prozac group decreased (P<0.05)3Compared with model group,pulse group,musical group can increase the rats’ weight at the end of14day(P<0.05);Compared with model group,pulse group,musical group and Prozac group can increase the rats’ weight (P<0.05). there was no significant change between Prozac group and pulse group(P>0.05); Compared with Prozac group and pulse group,musical group can increase the rats’ weight (P<0.05).4Compared with model group,pulse group,musical group and Prozac group can increase the rats’ sucrose intake (P<0.05), there was no difference among pulse group,musical group and Prozac group (P>0.05)5Compared with model group, the level ofGAS in colonic mucosa tissues and GAS,NPY,CGRP in hypothalamus tissues were increased significantly in musical group Prozac group and pulse group, while the level of NPY and CGRP in colonic mucosa tissues was reduced obviously (P<0.05). The music group was superior to the pulse group in adjusting the GAS, CGRP level of rat colonic mucosa tissues and the GAS,NPY level of hypothalamus tissues (P<0.05)6Compared with model group, the level of β-HP in hypothalamus tissues was increased significantly, the level of β-HP in colonic mucosa tissues was reduced obviously in musical group. Prozac group and pulse group(P<0.05)7Compared with model group,pulse group,musical group and Prozac group can increase the rats’ small intestinal propulsion rate (P<0.05), musical group was supurior than pulse group and Prozac group (P<0.05)Conclusion1Both pulse electro-acupuncture and musical electro-acupuncture can improve symptoms of depression of Liver stagnation and spleen deficiency syndrome,musical electro-acupuncture is better than pulse electro-acupuncture.2Animal model of chronic stress was established successlully.Chronie stress could change rats ’behavior, upsetexcitingand inhibitinggastrointestinal hormone levelsand bring mortorphological changes of rat hippocampal neurons.3Hffect of electroacupuncture treatment can achieve positive control drug Prozac.4Hlcctro-acupuncture improved the gastrointestinal dysfunction after depression by adjusting the unbalance of the exciting and inhibiting gastrointestinal hormonelevels in colonic mucosa to influence on the brain gut axiswith the musical electro-acupuncture much more better. |
PDF Full Text Request