Study On Inhibitory Effects And Mechanism Of QYHJ Decoction On Human Pancreatic Cancer Cell Line CFPAC-1 In Nude Mice

ObjectivesThe aim of this study was to investigate the inhibitory effects of QYHJ decoction on human pancreatic cancer cell line CFPAC-1 in nude mice,and its influence on extracellular factors,cell cycle,cancer related genes and signal transduction.Materials and Methods1.Reaseach on Inhibitory effects of QYHJ decoction on experimental pancreatic cancer cell line CFPAC-1 in nude miceHuman pancreatic cancer cell line CFPAC-1 burdened nude mice were randomized to receive QYHJ decoction at different doses and oral chemotherapeutics.Inhibitory rates of tumor growth in vivo were calculated.2.Research on mechanism of inhibitory effects of QYHJ decoction on experimental pancreatic cancer cell line CFPAC-1 in nude mice2.1 ELISA was used to detect serum extracellular factors such as VEGF,TNF-α,sVCAM-1,TGF-β1,IL-6 and IL-8 in nude mice.2.2 Flow cytometry technique was used to analyze transplanted tumors of nude mice. RT-PCR and quantitative PCR were used to amplify the gene of EphB2. Lentivirus interference vector was constructed to knockdown the targeted gene EphB2 for further study. Results1.Inhibitory effects of QYHJ decoction on experimental pancreatic cancer cell line CFPAC-1 in nude miceQYHJ decoction could inhibit the growth of human pancreatic cancer cell line CFPAC-1 in vivo.Tumor weight in the QYHJ groups was significantly lower than that in control group(P＜0.05).Inhibiting rate of tumor growth varied from 33 to 38%in different dose of QYHJ groups.2.mechanism of inhibitory effects of QYHJ decoction on experimental pancreatic cancer cell line CFPAC-1 in nude mice2.1 The expression of serum extracellular factors such as VEGF,TNF-α,sVCAM-1,TGF-β1,IL-6 and IL-8 in nude mice:The serum level of TNF-α, IL-6 and IL-8 in the QYHJ-treated group was significantly lower than that in the control group(P＜0.05),and these above factors had a negative correlation with the inhibitory rate.There were no significant difference among each groups for the serum level of VEGF,TGF-β1 and sVCAM-1(P＞0.05).2.2 Results of cytometry analyses in each group:S-phase arrest was detected in the tumors of QYHJ-treated groups:The proportion of cells in S phase was higher, and which in G2/M phase was lower accordingly,when compared with the control group(P＜0.05).2.3 The expression of EphB2 in transplanted tumor of each group:The expression level of EphB2 in QYHJ-treated group was 2-fold or more elevated than that in the control group.Cytometry analyses revealed that downregulation of EphB2 may promote apoptosis in the CFPAC-1 cell line. Conclusions1.QYHJ decoction may remarkablely inhibit the growth of human pancreatic cancer cell line CFPAC-1 in vivo,the pattern of manifestation is to induce the pancreatic cancer cells to be necrosis.2.The mechanism of inhibitory effects of QYHJ decoction may correlate with downregulation of the serum level of extracellular factors,S-phase arrest in the cell cycle induced by QYHJ decoction,and upregulation of EphB2.