Studys On Protective Effects Of Polysaccharide Of Aioe Barbadensis From Steams And Leaves On Focal Cerebral Ischemia Demage In Rats

Objective:To study and explore the neuroprotective effects and thefurther mechanism of polysaccharide of Aloe barbadensis(PAB) oncerebral ischemia in rats.Methods:In order to observe the neuroprotective effect of PAB oncerebral ischemia,the rats with incomplete cerebral ischemia were inducedwith fishing lines in the middle cerebral artery(MCA) and then wereexecuted at 24h after cerebral ischemia.It was observed the effect of PABon the changes of ethology and pathomorphology due to nerve injury ofrats with MCAO before and after treatments.HE staining was used toobserve the changes of histomorphology.Electronic microscopy was usedto study the ultrastructure of areas.It was detected the effects of PAB onprotein expression ofcysteinyaspartate specificprotease-3(Caspase-3),Cyclooxygenase-2(COX-2),Nuclear Factor Kappa B p65(NF-κB p65)and intercellular adhesion molecule-1(ICAM-1) with immunohistochemicalmethod.In addition,we observe the rate of cell apoptosis with flowcytometry.Results:PAB could make obviously pathomorphology of cerebralischemia better,improve change of ethology,decrease the rate of cellapoptosis of ischemic cerebral tissue,inhibit the expression of cysteinyaspartate specific protease-3(Caspase-3),Cyclooxygenase-2(COX-2),Nuclear Factor Kappa B p65(NF-κB p65) and intercellular adhesionmolecule-1(ICAM-1) protein in cerebral cortex to protect the nerves aftercerebral ischemic injury of rats.Conclusion:PAB had protecting effect on cerebral ischemia,themechanism of PAB against cerebral ischemia is related toanti-inflammatory action and inhibiting cell apoptosis.