Studies On The Antioxidant And Hepatoprotective Effects Of Aloe Barbadensis And Its Polysaccharide

There is an extensive evidence to implicate that free radical is related to origin and development of many diseases. The peroxidantion induced by free radical is one of the machanism of pathological injury in liver tissue. The degree of injury may be regulated by antioxidant. In consider of the toxicity and side-effect of chemosynthesis product, it is important to exploit effective component with low-toxicity or untoxicity from natural animal or plant.Aloe is recognised as a traditional medical plant for its broad pharmacological activities. In this paper we isolate aloe polysaccaride from fresh leave of Aloe Barbadensis by means of hot-water extracting. Then the antioxidant and hepatoprotective effects of aloe and its polysaccharide were investigated. The main conclusions are as followed:1. The result of HPCE showed that polysaccaride of Aloe Barbadensis is composed of mannose, trehalose, arabinose.2. We choosed spectrophotometer method to study the scavenging effect of aloe and its polysaccharide on reactive oxidative free radical. In vitro, it was shown that both crude juice of aloe (CJA) and aloe polysaccharide (AP) were found to have significant capacity of scavenging free radical in a concentration or dose-dependant manner. The rates of scavenging effects on hydroxyl free radical and superoxide anion free radical by CJA with the concentration of 100% or dosage of lOOul were 95.28% and 77.48% respectively. While the rates of scavenging effects on hydroxyl free radical and superoxide anion free radical by AP 20mg/mL were 42.60% and 84.96% respectively. In addition, a significant inhibition of malondialdehyde (MDA) generation spontaneously in liver homogenate is also oberserved in crude juice of aloe in vitro, but its polysaccharide has no effect to be identified .3. Mice were administered intragastric (ig) CJA and AP for 10 consecutive days. Compared with the normal group, ,mice administered with CJA showed a significantdecrease in MDA production in liver homogenate and a significant increase in the activity of SOD simultaenously. But aloe polysaccharide has no significant influence to be found.4. To observe the hepatoprotective effects of Aloe Barbadensis and its polysaccharide, mice were pretreated by means of intragastric or intraperitonelly injection. Seven days later, they were injected 0.1% (V:V) carbon tetracholoride (CCl4) intraperitonelly at the dose of 10mL/kg.bw which induce acute hepatic injury. We found that the glutamic-pyruvic transaminase (GPT) level in serum and the MDA cotent in liver tissue were significantly decreased. Furthermore, the deterious lesions of hepatic tissue microstructure and sub-microstructure such as cell narcrosis, inflammation, mitochondrial denaturing induced by CCl4 were marked improved. These results suggested that both CJA and AP have hepataprotective function. The hepataprotective effect is related to its antioxidant activity.These results will provide some reference for making more exploitation on aloe.